Journal ArticleDOI
Expression, purification, immunogenicity and protective efficacy of a recombinant nucleoside hydrolase from Leishmania donovani, a vaccine candidate for preventing cutaneous leishmaniasis
C. Patrick McAtee,Christopher A. Seid,Molly Hammond,Elissa M. Hudspeth,Brian Keegan,Zhuyun Liu,Junfei Wei,Bin Zhan,Raul Arjona-Sabido,Vladimir Cruz-Chan,Eric Dumonteil,Peter J. Hotez,Maria Elena Bottazzi,Maria Elena Bottazzi +13 more
TLDR
Mice immunized with the LdNH36 antigen in combination with the GLA-SE adjuvant and challenged with Leishmania mexicana showed significant reductions in parasite burden, confirming the protective efficacy of this vaccine candidate.About:
This article is published in Protein Expression and Purification.The article was published on 2017-02-01. It has received 11 citations till now. The article focuses on the topics: Leishmania mexicana & Leishmania donovani.read more
Citations
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Journal ArticleDOI
A Chimera Containing CD4+ and CD8+ T-Cell Epitopes of the Leishmania donovani Nucleoside Hydrolase (NH36) Optimizes Cross-Protection against Leishmania amazonesis Infection
Marcus Vinícius Alves-Silva,Dirlei Nico,Alexandre Morrot,Marcos Palatnik,Clarisa B. Palatnik-de-Sousa +4 more
TL;DR: The epitope presentation in a recombinant chimera optimizes immunogenicity and efficacy above the levels induced by the independent or admixed F1 and F3 domains and advanced in the design of a NH36 polytope vaccine capable of inducing cross-protection to cutaneous leishmaniasis.
Journal ArticleDOI
Not All Antigens Are Created Equally: Progress, Challenges, and Lessons Associated with Developing a Vaccine for Leishmaniasis.
Malcolm S. Duthie,Steven G. Reed +1 more
TL;DR: It is clear that the vaccine platform of choice can have a significant impact upon the level of protection induced by particular antigens, and some examples for which the vaccine system used has impacted the protective efficacy imparted are provided.
Journal ArticleDOI
Immunizations and vaccines: a decade of successes and reversals, and a call for 'vaccine diplomacy'.
TL;DR: Over the last decade, there has been additional excitement over the potential development and introduction of new vaccines to prevent highly lethal respiratory virus infections, as well as tuberculosis, malaria, HIV/AIDS and several neglected tropical diseases.
Journal ArticleDOI
The importance of nucleoside hydrolase enzyme (NH) in studies to treatment of Leishmania: A review.
TL;DR: The aim of this study is to discuss the research and development of new agents for the treatment of Leishmania, and to stimulate the formulation of new NH inhibitors, to eliminate leishmaniasis disease in infected individuals.
Journal ArticleDOI
Nucleoside Hydrolase NH 36: A Vital Enzyme for the Leishmania Genus in the Development of T-Cell Epitope Cross-Protective Vaccines.
TL;DR: The FML-saponin vaccine became the first licensed veterinary vaccine against leishmaniasis (Leishmune®) which reduced the incidence of human and canine VL in endemic areas and identified the most immunogenic NH36 domains and epitopes for PBMC of active human VL, cured or asymptomatic and DTH+ patients.
References
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Journal ArticleDOI
Nucleoside hydrolase from Crithidia fasciculata. Metabolic role, purification, specificity, and kinetic mechanism.
TL;DR: The nucleoside hydrolase discriminates against methanol attack from solvent during steady-state catalysis, indicating the participation of an enzyme-directed water nucleophile.
Journal ArticleDOI
Nucleoside hydrolase from Leishmania major. Cloning, expression, catalytic properties, transition state inhibitors, and the 2.5-a crystal structure.
TL;DR: The full-length cDNA for nucleosid hydrolase from Leishmania major was cloned and sequence analysis revealed that the L. major nucleoside hydrol enzyme shares 78% sequence identity with the nonspecific nucleosides fromCrithidia fasciculata.
Journal ArticleDOI
Nucleosidases from Leishmania donovani. Pyrimidine ribonucleosidase, purine ribonucleosidase, and a novel purine 2'-deoxyribonucleosidase.
G W Koszalka,T A Krenitsky +1 more
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Global Burden of Disease Study 2015 provides GPS for global health 2030
TL;DR: The Global Burden of Disease Study 2015 (GBD 2015) provides a vital link between the Millennium Development Goals (MDGs) and the sustainable development goals (SDGs) for 2016-30 as mentioned in this paper.
Journal ArticleDOI
Multiple detection of pathogens in ticks: development of a high throughput real time PCR chip used as a new epidemiologic investigative tool
Lorraine Michelet,Sabine Delannoy,Elodie Devillers,Gérald Umhang,Anna Aspán,Mikael Juremalm,Jan Chirico,F.J. van der Wal,Hein Sprong,TP Boye Pihl,Kirstine Klitgaard,Rene Bødker,Patrick Fach,Sara Moutailler +13 more
TL;DR: A new epidemiologic investigative tool which could detect high number of tick-borne pathogens by real time PCR is developed and represents a major improvement for surveillance and future epidemiological studies.
Related Papers (5)
From mouse to man: safety, immunogenicity and efficacy of a candidate leishmaniasis vaccine LEISH-F3+GLA-SE.
Rhea N. Coler,Malcolm S. Duthie,Kimberly A. Hofmeyer,Jeffery Guderian,Lakshmi Jayashankar,Julie Vergara,Tom Rolf,Ayesha Misquith,John D. Laurance,Vanitha S. Raman,H. Remy Bailor,Natasha Dubois Cauwelaert,Steven J. Reed,Aarthy C. Vallur,Michelle Favila,Mark T. Orr,Jill A. Ashman,Prakash Ghosh,Dinesh Mondal,Steven G. Reed +19 more