Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease
Scott J. Gratz,Alexander M. Cummings,Jennifer N. Nguyen,Danielle C. Hamm,Laura K. Donohue,Melissa M. Harrison,Jill Wildonger,Kate M. O'Connor-Giles +7 more
TLDR
A bacterial CRISPR RNA/Cas9 system is adapted to precisely engineer the Drosophila genome and it is reported that Cas9-mediated genomic modifications are efficiently transmitted through the germline.Abstract:
We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.read more
Citations
More filters
Journal ArticleDOI
Intimate functional interactions between TGS1 and the Smn complex revealed by an analysis of the Drosophila eye development.
Paolo Maccallini,Francesca Bavasso,Livia Scatolini,Elisabetta Bucciarelli,Gemma Noviello,Veronica Lisi,Valeria Palumbo,S. D'Angeli,Stefano Cacchione,Giovanni Cenci,Laura Ciapponi,James G. Wakefield,Maurizio Gatti,Grazia D. Raffa +13 more
TL;DR: The results suggest that the Drosophila eye model can be exploited for screens aimed at the identification of genes and drugs that modify the phenotypes elicited by Tgs1 and Smn deficiency, which could help to understand the molecular mechanisms underlying SMA pathogenesis and devise new therapies for this genetic disease.
Journal ArticleDOI
CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner
Miki Hanasaki,Hiroshi Masumoto +1 more
TL;DR: CRITGI is a unique gene regulation system to induce the genes on pTy1 in amino acid depletion medium and utilizes Ty1 transcription to create a chromatin environment favorable for the transcription of the genes as well as a selection marker gene activation.
Posted ContentDOI
Autoregulation clamps the synaptic membrane-remodeling machinery and promotes productive actin-dependent endocytosis
Steven J. Del Signore,Charlotte F. Kelley,Emily M. Messelaar,Tania Lemos,Michelle F Marchan,Markus Mund,Marko Kaksonen,Avital A. Rodal +7 more
TL;DR: This work found that collective interactions between the Drosophila PAZ proteins Nwk/FCHSD2, Dap160/Intersectin, and WASp relieve Nwk autoinhibition and promote robust membrane-coupled actin assembly in vitro.
DissertationDOI
Gen-Editierung von Photorezeptorgenen in der Grünalge Chlamydomonas reinhardtii mithilfe des CRISPR/Cas9-Systems
TL;DR: This study established zinc-finger and CRISPR/Cas9 nucleases for gene editing to target and inactivate the photoreceptor genes in C. reinhardtii.
Dissertation
Functions of the SLC36 transporter Pathetic in growth control of Drosophila sensory neurons
TL;DR: In this paper, the SLC36 transporter Pathetic (Path) was identified as a factor required for extreme dendrite growth in neurons and it was shown that loss of path function imposes an upper limit on neuron growth, and additionally reported that path likely limits overall neurite length rather than dendritic length alone.
References
More filters
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,F. A. Ran,David Benjamin Turitz Cox,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +10 more
TL;DR: Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
Journal ArticleDOI
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
Journal ArticleDOI
CRISPR provides acquired resistance against viruses in prokaryotes
Rodolphe Barrangou,Christophe Fremaux,Hélène Deveau,Melissa Richards,Patrick Boyaval,Sylvain Moineau,Dennis A. Romero,Philippe Horvath +7 more
TL;DR: It is found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences, and CRISPR provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.