Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease
Scott J. Gratz,Alexander M. Cummings,Jennifer N. Nguyen,Danielle C. Hamm,Laura K. Donohue,Melissa M. Harrison,Jill Wildonger,Kate M. O'Connor-Giles +7 more
TLDR
A bacterial CRISPR RNA/Cas9 system is adapted to precisely engineer the Drosophila genome and it is reported that Cas9-mediated genomic modifications are efficiently transmitted through the germline.Abstract:
We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.read more
Citations
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Posted ContentDOI
A heterochromatin-specific RNA export pathway facilitates piRNA production
Mostafa F. ElMaghraby,Peter Refsing Andersen,Peter Refsing Andersen,Florian Pühringer,Katharina Meixner,Thomas Lendl,Laszlo Tirian,Julius Brennecke +7 more
TL;DR: The findings indicate that the piRNA pathway bypasses nuclear RNA surveillance systems to achieve export of heterochromatic, unprocessed transcripts to the cytoplasm, a strategy also exploited by retroviruses.
Book ChapterDOI
Autophagy and cell death in the fly.
Charles Nelson,Eric H. Baehrecke +1 more
TL;DR: This work describes how to analyze autophagy and its relationship to cell death in Drosophila and describes the role of lipids, proteins, and organelles in this process.
Journal ArticleDOI
Nmf9 Encodes a Highly Conserved Protein Important to Neurological Function in Mice and Flies.
Shuxiao Zhang,K. Ross,Glen A. Seidner,Michael R. Gorman,Tiffany H. Poon,Xiaobo Wang,Elizabeth M. Keithley,Patricia N. Lee,Mark Q. Martindale,William J. Joiner,Bruce A. Hamilton +10 more
TL;DR: It is shown that ENU-induced and genome-edited null mutations in mice produce deficits in vestibular function, fear learning and circadian behavior, which correlated with Nmf9 expression in inner ear, amygdala, and suprachiasmatic nuclei.
Journal ArticleDOI
Why mammalian wound-healing researchers may wish to turn to Drosophila as a model.
TL;DR: The benefits and limitations of using Drosophila in wound‐healing research are discussed, especially presenting this organism as a promising tool for the identification of new therapeutic targets and drugs in this context.
Journal ArticleDOI
A toolset to study functions of Cytosolic non-specific dipeptidase 2 (CNDP2) using Drosophila as a model organism
Evgeniya N. Andreyeva,A. A. Ogienko,A. A. Ogienko,T. D. Dubatolova,Anastasiya L. Oshchepkova,Elena N. Kozhevnikova,Anton V. Ivankin,Gera A. Pavlova,S. A. Kopyl,Alexey V. Pindyurin,Alexey V. Pindyurin +10 more
TL;DR: The d CNDP2 gene is not essential for fly viability under standard laboratory conditions, and the subcellular localization pattern of dCNDP2 suggests that this protein might have roles in both the cytoplasm and the nucleus.
References
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Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,F. A. Ran,David Benjamin Turitz Cox,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +10 more
TL;DR: Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
Journal ArticleDOI
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
Journal ArticleDOI
CRISPR provides acquired resistance against viruses in prokaryotes
Rodolphe Barrangou,Christophe Fremaux,Hélène Deveau,Melissa Richards,Patrick Boyaval,Sylvain Moineau,Dennis A. Romero,Philippe Horvath +7 more
TL;DR: It is found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences, and CRISPR provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.