scispace - formally typeset
Open AccessJournal ArticleDOI

Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease

TLDR
A bacterial CRISPR RNA/Cas9 system is adapted to precisely engineer the Drosophila genome and it is reported that Cas9-mediated genomic modifications are efficiently transmitted through the germline.
Abstract
We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.

read more

Citations
More filters

Expanding the Known DNA-binding Specificity of Homeodomains for Utility in Customizable Sequence-specific Nucleases: A Dissertation

TL;DR: In this paper, the authors present a Table of Contents for Table 1 : Table of CONTENTS for Table 2 : Table 1.5.1.2.0.1
Journal ArticleDOI

Evolutionarily conserved function of the even-skipped ortholog in insects revealed by gene knock-out analyses in Gryllus bimaculatus.

TL;DR: In this article , the authors compared the developmental mechanisms of segmentation among insects with different modes of embryogenesis, and found that the Drosophila pair-rule gene, even-skipped (eve), contributes to initial segmental patterning.

An in vivo structure-function analysis of the pathogenesis of triosephosphate isomerase deficiency.

TL;DR: Using the genomic engineering system, several novel TPI alleles are generated that further support the hypothesis that a conformational change at the dimer interface is sufficient to elicit TPI deficiency and it is conclusively shown that gross TPI activity is not predictive of disease presence or severity.
Posted ContentDOI

CAMIO for deletion analysis of endogenous DNA sequences in multicellular organisms

TL;DR: This work created Cas9-mediated Arrayed Mutagenesis of Individual Offspring (CAMIO) to achieve high-throughput analysis of native DNA and demonstrates the ease and power of CAMIO by mapping 5’UTR sequences crucial for chinmo’s post-transcriptional regulation.
References
More filters
Journal ArticleDOI

A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.

TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI

Multiplex Genome Engineering Using CRISPR/Cas Systems

TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.

Multiplex Genome Engineering Using CRISPR/Cas Systems

TL;DR: Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
Journal ArticleDOI

RNA-Guided Human Genome Engineering via Cas9

TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
Journal ArticleDOI

CRISPR provides acquired resistance against viruses in prokaryotes

TL;DR: It is found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences, and CRISPR provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.
Related Papers (5)