Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease
Scott J. Gratz,Alexander M. Cummings,Jennifer N. Nguyen,Danielle C. Hamm,Laura K. Donohue,Melissa M. Harrison,Jill Wildonger,Kate M. O'Connor-Giles +7 more
TLDR
A bacterial CRISPR RNA/Cas9 system is adapted to precisely engineer the Drosophila genome and it is reported that Cas9-mediated genomic modifications are efficiently transmitted through the germline.Abstract:
We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.read more
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Innate behavior sequence progression by peptide-mediated interorgan crosstalk
Fabiana Heredia,Yanel A. Volonté,Yanel A. Volonté,Joana Pereirinha,Magdalena Fernandez-Acosta,Andreia P. Casimiro,Cláudia G. Belém,Filipe Viegas,Kohtaro Tanaka,Maite Arana,Gisele Antoniazzi Cardoso,Gisele Antoniazzi Cardoso,Andre Macedo,Malwina Kotowicz,Facundo H. Prado Spalm,Marcos J. Dibo,Raquel Dietsche Monfardini,Raquel Dietsche Monfardini,Tatiana Teixeira Torres,César S. Mendes,Andres Garelli,Andres Garelli,Alisson M. Gontijo +22 more
TL;DR: It is found that the steroid-hormone ecdysone triggers parallel pupariation neuromotor and morphogenetic subprograms, which include the induction of the relaxin-peptide hormone, Dilp8, in the epidermis.
Mechanism and engineering of CRISPR-associated endonucleases
TL;DR: It is revealed that Cas9 can be programmed to target single-stranded RNA substrates for both high-affinity binding and site- specific cleavage using PAM-presenting oligonucleotides and that RNA targeting by Cas9 has the potential to transform the study of RNA function, much as site-specific DNA targeting has revolutionized genetic and genomic research.
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Maximum likelihood estimation of fitness components in experimental evolution
Jingxian Liu,Jackson Champer,Chen Liu,Joan Chung,Riona Reeves,Anisha Luthra,Yoo Lim Lee,Andrew G. Clark,Philipp W. Messer +8 more
TL;DR: A flexible maximum likelihood framework is developed that can disentangle different components of fitness and estimate them individually in males and females from genotype frequency data and should be generally applicable to situations where it is important to quantify fitness components of specific genetic variants.
References
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Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,F. A. Ran,David Benjamin Turitz Cox,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +10 more
TL;DR: Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
Journal ArticleDOI
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
Journal ArticleDOI
CRISPR provides acquired resistance against viruses in prokaryotes
Rodolphe Barrangou,Christophe Fremaux,Hélène Deveau,Melissa Richards,Patrick Boyaval,Sylvain Moineau,Dennis A. Romero,Philippe Horvath +7 more
TL;DR: It is found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences, and CRISPR provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.