Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease
Scott J. Gratz,Alexander M. Cummings,Jennifer N. Nguyen,Danielle C. Hamm,Laura K. Donohue,Melissa M. Harrison,Jill Wildonger,Kate M. O'Connor-Giles +7 more
TLDR
A bacterial CRISPR RNA/Cas9 system is adapted to precisely engineer the Drosophila genome and it is reported that Cas9-mediated genomic modifications are efficiently transmitted through the germline.Abstract:
We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.read more
Citations
More filters
Dissertation
Physiologie des récepteurs gustatifs chez la mouche de vinaigre (Drosophila melanogaster)
TL;DR: En utilisant des constructions genetiques et en particulier le systeme UAS-Gal4, nous avons montre that the Lcanavanine est detectee par des cellules gustatives qui expriment une proteine receptrice GR66a, qui est impliquee dans the detection of nombreuses substances ameres.
Book ChapterDOI
CRISPR/Cas-Based Insect Resistance in Crops
Muhammad Kashif Zahoor,Aftab Ahmad,Muhammad Asif Zahoor,Humara Naz Majeed,Muhammad Zulhussnain,Kanwal Ranian +5 more
TL;DR: In this article, a CRISPR-associated system (CRISPR/Cas) based genome editing and gene drives are becoming available for insecticide resistance management in crop production.
Posted ContentDOI
Genome engineering of Nannochloropsis with large deletions for constructing microalgal minigenomes
Qintao Wang,Yanhai Gong,Yuehui He,Yi Xin,Nana Lv,Xuefeng Du,Yun Li,Byeong-ryool Jeong,Jian Xu +8 more
TL;DR: A method to precisely and serially delete large genome fragments of ~100 kb from its 30.01-Mb nuclear genome is established, which will accelerate crafting minimal genomes as chassis for photosynthetic production.
Journal ArticleDOI
Enhancer analysis of the Drosophila zinc finger transcription factor Earmuff by gene targeting.
Kirsten Hildebrandt,Sabrina Kübel,Marie Minet,Nora Fürst,Christine Klöppel,Eva Louise Steinmetz,Uwe Walldorf +6 more
TL;DR: In this article, a functional analysis of erm and some of its enhancers was performed and the deletion of three of the previously analysed enhancers showing the most prominent expression patterns of Erm by gene targeting resulted in specific temporal and spatial defects in defined brain structures.
Journal ArticleDOI
The microRNA miR-33 is a pleiotropic regulator of metabolic and developmental processes in Drosophila melanogaster.
Laure-Alix Clerbaux,Laure-Alix Clerbaux,Hayley Schultz,Samara Roman-Holba,Dan Fu Ruan,Ronald Yu,Abigail M. Lamb,Guido T. Bommer,Jennifer A. Kennell +8 more
TL;DR: In this paper, the effects of miR-33 deficiency on lipid metabolism and other phenotypes impacted by metabolic state have not yet been characterized, but it has been shown that miR33 is a conserved regulator of lipid homeostasis and its targets are involved in both degradation and synthesis of fatty acids and TAG.
References
More filters
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,F. A. Ran,David Benjamin Turitz Cox,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +10 more
TL;DR: Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
Journal ArticleDOI
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
Journal ArticleDOI
CRISPR provides acquired resistance against viruses in prokaryotes
Rodolphe Barrangou,Christophe Fremaux,Hélène Deveau,Melissa Richards,Patrick Boyaval,Sylvain Moineau,Dennis A. Romero,Philippe Horvath +7 more
TL;DR: It is found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences, and CRISPR provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.