Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease
Scott J. Gratz,Alexander M. Cummings,Jennifer N. Nguyen,Danielle C. Hamm,Laura K. Donohue,Melissa M. Harrison,Jill Wildonger,Kate M. O'Connor-Giles +7 more
TLDR
A bacterial CRISPR RNA/Cas9 system is adapted to precisely engineer the Drosophila genome and it is reported that Cas9-mediated genomic modifications are efficiently transmitted through the germline.Abstract:
We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.read more
Citations
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Journal ArticleDOI
Demonstration of a Simple Epitope Tag Multimerization Strategy for Enhancing the Sensitivity of Protein Detection Using Drosophila vAChT.
TL;DR: Conditional Drosophila vAChT alleles containing two tandem copies of epitope tags are generated using the repeat multimerization method, which is effective for DNA repeats of at least 56 bp and should be generally applicable to any species.
Book ChapterDOI
Advances in Functional Genomics in Investigating Salinity Tolerance in Plants
TL;DR: This chapter specifically describes the over-expression or down-regulation of different candidate genes for mitigating salt tolerance in plants and summarizes how genome editing is helpful in controlling the detrimental effects of salt stress.
Book ChapterDOI
Engineered Minichromosomes in Plants: Structure, Function, and Applications
Nathaniel D. Graham,Jon P. Cody,Nathan C. Swyers,Morgan E. McCaw,Changzeng Zhao,James A. Birchler +5 more
TL;DR: Of the two methods proposed for creating engineered minichromosomes, telomere-mediated truncation is more reliable in plant systems and the creation of minichROMosomes with binary bacterial artificial chromosome vectors provides the ability to introduce many transgenes at one time.
Journal ArticleDOI
The human cognition-enhancing CORD7 mutation increases active zone number and synaptic release.
Mila M. Paul,Sven Dannhäuser,Lydia Morris,Achmed Mrestani,Martha E. Hübsch,Jennifer Gehring,Georgios N. Hatzopoulos,Martin Pauli,Genevieve M Auger,Grit Bornschein,Nicole Scholz,Dmitrij Ljaschenko,Martin Müller,Markus Sauer,Hartmut Schmidt,Robert J. Kittel,Aaron DiAntonio,Ioannis Vakonakis,Manfred Heckmann,Tobias Langenhan +19 more
TL;DR: Drosophila melanogaster is established as a disease model for clarifying the effects of the CORD7 mutation on RIM function and synaptic vesicle release and it is concluded that the Cord7 mutation leads to tighter release coupling, an increased readily releasable pool size and more release sites thereby promoting more efficient synaptic transmitter release.
RNA interference of worker sterility genes: Testing mechanisms of reproductive regulation in Apis mellifera
TL;DR: The aim of this study was to establish a database of lay audience beliefs andatos and establish a procedure for cataloguing and quantifying these beliefs.
References
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Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,F. A. Ran,David Benjamin Turitz Cox,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +10 more
TL;DR: Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
Journal ArticleDOI
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
Journal ArticleDOI
CRISPR provides acquired resistance against viruses in prokaryotes
Rodolphe Barrangou,Christophe Fremaux,Hélène Deveau,Melissa Richards,Patrick Boyaval,Sylvain Moineau,Dennis A. Romero,Philippe Horvath +7 more
TL;DR: It is found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences, and CRISPR provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.