Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease
Scott J. Gratz,Alexander M. Cummings,Jennifer N. Nguyen,Danielle C. Hamm,Laura K. Donohue,Melissa M. Harrison,Jill Wildonger,Kate M. O'Connor-Giles +7 more
TLDR
A bacterial CRISPR RNA/Cas9 system is adapted to precisely engineer the Drosophila genome and it is reported that Cas9-mediated genomic modifications are efficiently transmitted through the germline.Abstract:
We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.read more
Citations
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Creation of Early Flowering Germplasm of Soybean by CRISPR/Cas9 Technology.
TL;DR: A photo-insensitive soybean transformation receptor was created that laid a foundation for breeding excellent transgenic receptors suitable for high latitudes by introducing the CRISPR/Cas9 expression vector into soybean cultivar Jack and generating targeted mutants of E1 gene controlling soybean flowering.
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Robust CRISPR/Cas9-Mediated Tissue-Specific Mutagenesis Reveals Gene Redundancy and Perdurance in Drosophila
Amy R Poe,Bei Wang,Maria L. Sapar,Hui Ji,Kailyn Li,Tireniolu Onabajo,Rushaniya Fazliyeva,Mary Gibbs,Yue Qiu,Yuzhao Hu,Chun Han +10 more
TL;DR: It is shown that insertions or deletions in coding sequences more reliably cause somatic mutations than DNA excisions induced by two gRNAs, and CRISPR-TRiM efficiently unmasks redundant soluble N-ethylmaleimide–sensitive factor attachment protein receptor gene functions in neurons and epidermal cells.
Journal ArticleDOI
A chemical-genetics approach to study the role of atypical Protein Kinase C in Drosophila.
TL;DR: The generation of an analogue-sensitive allele of atypical Protein Kinase C in Drosophila allows dissection of the function of aPKC kinase activity with high specificity and temporal control.
Journal ArticleDOI
A technological and regulatory outlook on CRISPR crop editing.
Rea Globus,Udi Qimron +1 more
TL;DR: The challenges facing genetic engineering of crops by CRISPR‐cas are discussed, and the pros and cons of using this tool are highlighted.
Journal ArticleDOI
Effect of Genetic Variation in a Drosophila Model of Diabetes-Associated Misfolded Human Proinsulin
Bin Z. He,Michael Ludwig,Desiree A. Dickerson,Levi Barse,Bharath Arun,Bjarni J. Vilhjálmsson,Pengyao Jiang,Soo-Young Park,Natalia A. Tamarina,Scott B. Selleck,Patricia J. Wittkopp,Graeme I. Bell,Martin Kreitman +12 more
TL;DR: The ability to create a model of human genetic disease, map a QTL by GWAS to a specific gene, and validate its contribution to disease with available genetic resources and the potential to experimentally link the variant to a molecular mechanism demonstrate the many advantages Drosophila holds in determining the genetic underpinnings of human disease.
References
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Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,F. A. Ran,David Benjamin Turitz Cox,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +10 more
TL;DR: Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
Journal ArticleDOI
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
Journal ArticleDOI
CRISPR provides acquired resistance against viruses in prokaryotes
Rodolphe Barrangou,Christophe Fremaux,Hélène Deveau,Melissa Richards,Patrick Boyaval,Sylvain Moineau,Dennis A. Romero,Philippe Horvath +7 more
TL;DR: It is found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences, and CRISPR provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.