Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease
Scott J. Gratz,Alexander M. Cummings,Jennifer N. Nguyen,Danielle C. Hamm,Laura K. Donohue,Melissa M. Harrison,Jill Wildonger,Kate M. O'Connor-Giles +7 more
TLDR
A bacterial CRISPR RNA/Cas9 system is adapted to precisely engineer the Drosophila genome and it is reported that Cas9-mediated genomic modifications are efficiently transmitted through the germline.Abstract:
We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.read more
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Applying functional genomics to the study of lamprey development and sea lamprey population control
TL;DR: How functional genetic, transcriptomic, and CRISPR/Cas9 genome editing technologies in lampreys may be repurposed for use by fishery and conservation biologists to approach the problem of invasive sea lamprey from a molecular-genetic perspective is described.
Posted ContentDOI
An autoinhibitory clamp of actin assembly constrains and directs synaptic endocytosis
Steven J. Del Signore,Charlotte F. Kelley,Emily M. Messelaar,Tania Lemos,Michelle F Marchan,Biljana Ermanoska,Markus Mund,Thomas G. Fai,Marko Kaksonen,Avital A. Rodal +9 more
TL;DR: Using automated particle tracking to quantify synaptic actin dynamics in vivo, it is discovered that Nwk-Dap160 interactions constrain spurious assembly of WASp-dependent actin structures, suggesting that autoinhibition both clamps and primes the synaptic endocytic machinery, thereby constraining actin assembly to drive productive membrane remodeling in response to physiological cues.
Journal ArticleDOI
Genetically Encoded CRISPR Components Yield Efficient Gene Editing in the Invasive Pest Drosophila suzukii.
Nikolay P. Kandul,Esther J. Belikoff,Junru Liu,Anna Buchman,Fang Li,Akihiko Yamamoto,Ting Yang,Isaiah Shriner,Maxwell J. Scott,Omar S. Akbari +9 more
TL;DR: The Drosophila suzukii Matsumura is a global pest of economically important soft-skinned fruits and it is largely controlled through r... as mentioned in this paper, but it is difficult to control.
Journal ArticleDOI
Narya, a RING finger domain-containing protein, is required for meiotic DNA double-strand break formation and crossover maturation in Drosophila melanogaster.
Cathleen M. Lake,Rachel J. Nielsen,Amanda M. Bonner,Salam Eche,Sanese K. White-Brown,Kim S. McKim,R. Scott Hawley,R. Scott Hawley +7 more
TL;DR: The view that all three RING finger domain-containing proteins function in the formation of meiotic DNA DSBs and in the process of crossing over is supported.
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The making of an olfactory specialist
Thomas O. Auer,Mohammed A. Khallaf,Ana F. Silbering,Giovanna Zappia,Kaitlyn Ellis,Bill S. Hansson,Gregory S.X.E. Jefferis,Sophie Jeanne Cécile Caron,Markus Knaden,Richard Benton +9 more
TL;DR: A novel neurogenetic model is developed, Drosophila sechellia, a close cousin of D. melanogaster that displays profound behavioural changes linked to its extreme host fruit specialisation that defines a powerful model for investigating nervous system evolution and speciation.
References
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Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,F. A. Ran,David Benjamin Turitz Cox,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +10 more
TL;DR: Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
Journal ArticleDOI
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
Journal ArticleDOI
CRISPR provides acquired resistance against viruses in prokaryotes
Rodolphe Barrangou,Christophe Fremaux,Hélène Deveau,Melissa Richards,Patrick Boyaval,Sylvain Moineau,Dennis A. Romero,Philippe Horvath +7 more
TL;DR: It is found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences, and CRISPR provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.