Human haematopoietic stem cell development: from the embryo to the dish
Andrejs Ivanovs,Stanislav Rybtsov,Elizabeth Ng,Elizabeth Ng,Edouard G. Stanley,Edouard G. Stanley,Edouard G. Stanley,Andrew G. Elefanty,Alexander Medvinsky +8 more
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TLDR
The extent to which in vitro differentiation of human pluripotent stem cells recapitulates bona fide human developmental haematopoiesis is discussed, and some future directions in the field are outlined.Abstract:
Haematopoietic stem cells (HSCs) emerge during embryogenesis and give rise to the adult haematopoietic system. Understanding how early haematopoietic development occurs is of fundamental importance for basic biology and medical sciences, but our knowledge is still limited compared with what we know of adult HSCs and their microenvironment. This is particularly true for human haematopoiesis, and is reflected in our current inability to recapitulate the development of HSCs from pluripotent stem cells in vitro In this Review, we discuss what is known of human haematopoietic development: the anatomical sites at which it occurs, the different temporal waves of haematopoiesis, the emergence of the first HSCs and the signalling landscape of the haematopoietic niche. We also discuss the extent to which in vitro differentiation of human pluripotent stem cells recapitulates bona fide human developmental haematopoiesis, and outline some future directions in the field.read more
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Gastruloids as in vitro models of embryonic blood development with spatial and temporal resolution
TL;DR: Gastruloids are three-dimensional embryonic organoids that reproduce key features of early mammalian development in vitro with unique scalability, accessibility, and spatiotemporal similarity to real embryos as discussed by the authors .
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Vast Self-Renewal Potential of Human AGM Region HSCs Dramatically Declines in the Umbilical Cord Blood.
TL;DR: It is quantitatively shown that a single human AGM region HSC can generate 600–1,600 functional daughter HSCs, which sets a new bar for generation of clinically useful H SCs from pluripotent stem cells.
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Industrially Compatible Transfusable iPSC-Derived RBCs: Progress, Challenges and Prospective Solutions
Zhong Ri Lim,Svetlan Vassilev,Yew Wai Leong,Yew Wai Leong,Jing Wen Hang,Laurent Rénia,Laurent Rénia,Benoit Malleret,Benoit Malleret,Steve Oh +9 more
TL;DR: In this paper, the major challenges and prospective solutions throughout the whole process of erythropoiesis for hiPSC-derived red blood cells (RBCs) are highlighted.
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Biomechanical Regulation of Hematopoietic Stem Cells in the Developing Embryo
Paulina D. Horton,Sandeep P. Dumbali,Krithikaa Rajkumar Bhanu,Miguel F. Diaz,Pamela L. Wenzel,Pamela L. Wenzel +5 more
TL;DR: In this article, the biomechanics of the endothelial-to-hematopoietic transition (EHT), impact of force on organelles, and signaling triggered by extrinsic forces within the aorta-gonad-mesonephros (AGM), the primary site of HSC emergence.
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Tipping the Scales With Zebrafish to Understand Adaptive Tumor Immunity
TL;DR: In this paper, the authors provide an overview of the development of the zebrafish immune system along with a side-by-side comparison of its human counterpart and introduce components of the adaptive immune system with a focus on their roles in the tumor microenvironment (TME) of teleosts.
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Journal Article
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