Human haematopoietic stem cell development: from the embryo to the dish
Andrejs Ivanovs,Stanislav Rybtsov,Elizabeth Ng,Elizabeth Ng,Edouard G. Stanley,Edouard G. Stanley,Edouard G. Stanley,Andrew G. Elefanty,Alexander Medvinsky +8 more
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TLDR
The extent to which in vitro differentiation of human pluripotent stem cells recapitulates bona fide human developmental haematopoiesis is discussed, and some future directions in the field are outlined.Abstract:
Haematopoietic stem cells (HSCs) emerge during embryogenesis and give rise to the adult haematopoietic system. Understanding how early haematopoietic development occurs is of fundamental importance for basic biology and medical sciences, but our knowledge is still limited compared with what we know of adult HSCs and their microenvironment. This is particularly true for human haematopoiesis, and is reflected in our current inability to recapitulate the development of HSCs from pluripotent stem cells in vitro In this Review, we discuss what is known of human haematopoietic development: the anatomical sites at which it occurs, the different temporal waves of haematopoiesis, the emergence of the first HSCs and the signalling landscape of the haematopoietic niche. We also discuss the extent to which in vitro differentiation of human pluripotent stem cells recapitulates bona fide human developmental haematopoiesis, and outline some future directions in the field.read more
Citations
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From haematopoietic stem cells to complex differentiation landscapes
Elisa Laurenti,Berthold Göttgens +1 more
TL;DR: These evolving views of haematopoiesis have broad implications for the understanding of the functions of adult stem cells, as well as the development of new therapies for malignant and non-malignant haem atopoietic diseases.
Journal ArticleDOI
Decoding human fetal liver haematopoiesis
Dorin-Mirel Popescu,Rachel A. Botting,Emily Stephenson,Kile Green,Simone Webb,Laura Jardine,Emily Calderbank,Krzysztof Polanski,Issac Goh,Mirjana Efremova,Meghan Acres,Daniel Maunder,Peter Vegh,Yorick Gitton,Jong-Eun Park,Roser Vento-Tormo,Zhichao Miao,Zhichao Miao,David Dixon,Rachel Rowell,David McDonald,James Fletcher,Elizabeth Poyner,Gary Reynolds,Michael W. Mather,Corina Moldovan,Lira Mamanova,Frankie Greig,Matthew D. Young,Kerstin B. Meyer,Steven Lisgo,Jaume Bacardit,Andrew Fuller,Ben Millar,Barbara A. Innes,Susan Lindsay,Michael J. T. Stubbington,Monika S. Kowalczyk,Bo Li,Orr Ashenberg,Marcin Tabaka,Danielle Dionne,Timothy L. Tickle,Timothy L. Tickle,Michal Slyper,Orit Rozenblatt-Rosen,Andrew Filby,Peter D. Carey,Alexandra-Chloé Villani,Alexandra-Chloé Villani,Anindita Roy,Aviv Regev,Aviv Regev,Alain Chédotal,Irene Roberts,Berthold Göttgens,Sam Behjati,Sam Behjati,Elisa Laurenti,Sarah A. Teichmann,Sarah A. Teichmann,Muzlifah Haniffa,Muzlifah Haniffa +62 more
TL;DR: A shift in the haemopoietic composition of fetal liver during gestation away from being predominantly erythroid, accompanied by a parallel change in differentiation potential of HSC/MPPs is demonstrated, which is validated to produce an integrated map of fetal liver haematopoiesis.
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High-Spatial-Resolution Multi-Omics Sequencing via Deterministic Barcoding in Tissue
Yang Liu,Mingyu Yang,Yanxiang Deng,Graham Su,Archibald Enninful,Cindy C. Guo,Toma Tebaldi,Di Zhang,Dong-Joo Kim,Zhiliang Bai,Eileen Norris,Alisia Pan,Jiatong Li,Yang Xiao,Stephanie Halene,Rong Fan +15 more
TL;DR: D deterministic barcoding in tissue for spatial omics sequencing (DBiT-seq) is presented for co-mapping of mRNAs and proteins in a formaldehyde-fixed tissue slide via next-generation sequencing (NGS) and can be adopted by researchers with no experience in microfluidics.
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Biologic properties and enucleation of red blood cells from human embryonic stem cells. Commentary
Eric E. Bouhassira,Shi-Jiang Lu,Qiang Feng,Jennifer S. Park,Loyda N. Vida,Bao-Shiang Lee,Michael A. Strausbauch,Peter J. Wettstein,George R. Honig,Robert Lanza +9 more
TL;DR: It is shown that it is feasible to differentiate and mature human embryonic stem cells (hESCs) into functional oxygen-carrying erythrocytes on a large scale (10(10)-10(11) cells/6-well plate hESCs).
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Deciphering human macrophage development at single-cell resolution
Zhilei Bian,Yandong Gong,Tao Huang,Christopher Z. W. Lee,Christopher Z. W. Lee,Lihong Bian,Zhijie Bai,Hui Shi,Yang Zeng,Chen Liu,Jian He,Jie Zhou,Xianlong Li,Zongcheng Li,Yanli Ni,Chunyu Ma,Lei Cui,Rui Zhang,Jerry Kok Yen Chan,Jerry Kok Yen Chan,Lai Guan Ng,Yu Lan,Florent Ginhoux,Bing Liu,Bing Liu,Bing Liu +25 more
TL;DR: The data represent a comprehensive characterization of the spatiotemporal dynamics of early macrophage development during human embryogenesis, providing a reference for future studies of the development and function of human TRMs.
References
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