IGF-1 enhances cell proliferation and survival during early differentiation of mesenchymal stem cells to neural progenitor-like cells
Tee Jong Huat,Amir Ali Khan,Amir Ali Khan,Soumya Pati,Soumya Pati,Zulkifli Mustafa,Jafri Malin Abdullah,Hasnan Jaafar +7 more
TLDR
It is suggested that IGF-1 has a crucial role in the differentiation of MSCs into neuronal lineage by enhancing the proliferation and reducing the apoptosis in the NPCs.Abstract:
There has been increasing interest recently in the plasticity of mesenchymal stem cells (MSCs) and their potential to differentiate into neural lineages. To unravel the roles and effects of different growth factors in the differentiation of MSCs into neural lineages, we have differentiated MSCs into neural lineages using different combinations of growth factors. Based on previous studies of the roles of insulin-like growth factor 1 (IGF-1) in neural stem cell isolation in the laboratory, we hypothesized that IGF-1 can enhance proliferation and reduce apoptosis in neural progenitor-like cells (NPCs) during differentiation of MSCs into NCPs. We induced MSCs differentiation under four different combinations of growth factors: (A) EGF + bFGF, (B) EGF + bFGF + IGF-1, (C) EGF + bFGF + LIF, (D) EGF + bFGF + BDNF, and (E) without growth factors, as a negative control. The neurospheres formed were characterized by immunofluorescence staining against nestin, and the expression was measured by flow cytometry. Cell proliferation and apoptosis were also studied by MTS and Annexin V assay, respectively, at three different time intervals (24 hr, 3 days, and 5 days). The neurospheres formed in the four groups were then terminally differentiated into neuron and glial cells. The four derived NPCs showed a significantly higher expression of nestin than was shown by the negative control. Among the groups treated with growth factors, NPCs treated with IGF-1 showed the highest expression of nestin. Furthermore, NPCs derived using IGF-1 exhibited the highest cell proliferation and cell survival among the treated groups. The NPCs derived from IGF-1 treatment also resulted in a better yield after the terminal differentiation into neurons and glial cells than that of the other treated groups. Our results suggested that IGF-1 has a crucial role in the differentiation of MSCs into neuronal lineage by enhancing the proliferation and reducing the apoptosis in the NPCs. This information will be beneficial in the long run for improving both cell-based and cell-free therapy for neurodegenerative diseases.read more
Citations
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The role of Insulin-Like Growth Factor 1 (IGF-1) in brain development, maturation and neuroplasticity.
TL;DR: The role of IGF-1 in brain development is reviewed, followed by a detailed discussion of the role played by IGF in cellular neuroplasticity in the CNS.
Journal ArticleDOI
BDNF as a Promising Therapeutic Agent in Parkinson's Disease.
Ewelina Palasz,Adrianna Wysocka,Anna Gasiorowska,Małgorzata Chalimoniuk,Wiktor Niewiadomski,Grazyna Niewiadomska +5 more
TL;DR: Evidence is focused on the evidence that increasing BDNF level due to gene modulation or physical exercise has a neuroprotective effect and could be considered as adjunctive therapy in PD.
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Biomarkers of postoperative delirium and cognitive dysfunction.
TL;DR: Application of integrated systems biology has the potential to reconstruct the underlying network of molecular mechanisms and help in the identification of prognostic and diagnostic biomarkers.
Journal ArticleDOI
The Roles of Insulin-Like Growth Factors in Mesenchymal Stem Cell Niche.
TL;DR: The importance of IGFs, IGF-1 and IGF-2, in development and in the MSC niche and how they affect the pluripotency or differentiation towards multiple lineages of the three germ layers are described.
Journal ArticleDOI
Mesenchymal stem cell therapy for the treatment of traumatic brain injury: progress and prospects.
TL;DR: In this article, a review of mesenchymal stem cells (MSC) after Traumatic Brain Injury (TBI) and its future direction is presented. But, MSC therapy has a few drawbacks including tumor formation, which can be avoided by the use of MSC-derived exosomes.
References
More filters
Journal ArticleDOI
Mesenchymal and haematopoietic stem cells form a unique bone marrow niche
Simón Méndez-Ferrer,Tatyana V. Michurina,Francesca Ferraro,Amin R. Mazloom,Ben D. MacArthur,Ben D. MacArthur,Sergio A. Lira,David T. Scadden,Avi Ma'ayan,Grigori Enikolopov,Paul S. Frenette,Paul S. Frenette +11 more
TL;DR: It is demonstrated that mesenchymal stem cells (MSCs), identified using nestin expression, constitute an essential HSC niche component and are indicative of a unique niche in the bone marrow made of heterotypic stem-cell pairs.
Journal ArticleDOI
Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood.
Wolfgang Wagner,Frederik Wein,Anja Seckinger,Maria Frankhauser,Ute Wirkner,Ulf Krause,Jonathon Blake,Christian Schwager,Volker Eckstein,Wilhelm Ansorge,Anthony D. Ho +10 more
TL;DR: The results have provided a foundation for a more reproducible and reliable quality control using genotypic analysis for defining MSC.
Journal ArticleDOI
A long-term follow-up study of intravenous autologous mesenchymal stem cell transplantation in patients with ischemic stroke.
TL;DR: Intravenous autologous MSCs transplantation was safe for stroke patients during long‐term follow‐up and may improve recovery after stroke depending on the specific characteristics of the patients.
Journal ArticleDOI
Specific induction of neuronal cells from bone marrow stromal cells and application for autologous transplantation
Mari Dezawa,Hiroshi Kanno,Mikio Hoshino,Hirotomi Cho,Naoya Matsumoto,Yutaka Itokazu,Nobuyoshi Tajima,Hitoshi Yamada,Hajime Sawada,Hiroto Ishikawa,Toshirou Mimura,Masaaki Kitada,Yoshihisa Suzuki,Chizuka Ide +13 more
TL;DR: It is shown that a population of neuronal cells can be specifically generated from MSCs and that induced cells may allow for a neuroreconstructive approach.
Journal ArticleDOI
Efficient generation of neural stem cell-like cells from adult human bone marrow stromal cells.
Andreas Hermann,Regina Gastl,Stefan Liebau,M. Oana Popa,Jörg Fiedler,Bernhard O. Boehm,Martina Maisel,Holger Lerche,Johannes Schwarz,Johannes Schwarz,Rolf E. Brenner,Alexander Storch +11 more
TL;DR: The efficient conversion of human adult bone marrow stromal cells (hMSC) into a neural stem cell-like population (hmNSC, for human marrow-derived NSC-like cells) is described and it is demonstrated that individual hmNSCs are multipotent and retain the capacity to generate both glia and neurons.