Journal ArticleDOI
Isolation of autophagocytosis mutants of Saccharomyces cerevisiae.
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TLDR
The isolation of yeast mutants defective in autophagocytosis (aut mutants) using a rapid colony screening procedure, and the elucidation of its mechanisms via genetic and molecular biological investigations are reported.About:
This article is published in FEBS Letters.The article was published on 1994-08-01. It has received 573 citations till now. The article focuses on the topics: Protein degradation & Saccharomyces cerevisiae.read more
Citations
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Journal ArticleDOI
Autophagy as a Regulated Pathway of Cellular Degradation
Daniel J. Klionsky,Scott D. Emr +1 more
TL;DR: The core protein machinery that is necessary to drive formation and consumption of intermediates in the macroautophagy pathway includes a ubiquitin-like protein conjugation system and a protein complex that directs membrane docking and fusion at the lysosome or vacuole.
Journal ArticleDOI
Promotion of tumorigenesis by heterozygous disruption of the beclin 1 autophagy gene
Xueping Qu,Jie Yu,Govind Bhagat,Norihiko Furuya,Hanina Hibshoosh,Andrea B. Troxel,Jeffrey M. Rosen,Eeva-Liisa Eskelinen,Noboru Mizushima,Yoshinori Ohsumi,Giorgio Cattoretti,Beth Levine +11 more
TL;DR: It is shown that heterozygous disruption of beclin 1 increases the frequency of spontaneous malignancies and accelerates the development of hepatitis B virus-induced premalignant lesions, providing genetic evidence that autophagy is a novel mechanism of cell-growth control and tumor suppression.
Journal ArticleDOI
Self-consumption: the interplay of autophagy and apoptosis
TL;DR: The dialogue between autophagy and cell death pathways influences the normal clearance of dying cells, as well as immune recognition of dead cell antigens, and the disruption of the relationship between autphagy and apoptosis has important pathophysiological consequences.
Journal ArticleDOI
The machinery of macroautophagy
TL;DR: This review focuses on macroautophagy, briefly describing the discovery of this process in mammalian cells, discussing the current views concerning the donor membrane that forms the phagophore, and characterizing the autophagy machinery including the available structural information.
Journal ArticleDOI
Dynamics and diversity in autophagy mechanisms: lessons from yeast
TL;DR: The discovery of autophagy in yeast and the genetic tractability of this organism have allowed us to identify genes that are responsible for this process, which has led to the explosive growth of this research field seen today.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae.
Miki Tsukada,Yoshinori Ohsumi +1 more
TL;DR: The results on the apg mutants suggest that autophagy via autophagic bodies is indispensable for protein degradation in the vacuoles under starvation conditions, and that at least 15 APG genes are involved inAutophagy in yeast.
Journal ArticleDOI
Autophagy in yeast demonstrated with proteinase-deficient mutants and conditions for its induction
TL;DR: Accumulation of autophagic bodies in the vacuoles was induced not only by nitrogen starvation, but also by depletion of nutrients such as carbon and single amino acids that caused cessation of the cell cycle.
Book ChapterDOI
Immunofluorescence Methods for Yeast
TL;DR: This chapter provides protocols for the application of immunofluorescence procedures to yeast and notes especially the great labor involved in visualizing the structure of whole cells by serial-section methods.
Journal ArticleDOI
Peptide sequences that target cytosolic proteins for lysosomal proteolysis
TL;DR: One mechanism by which proteins enter lysosomes for subsequent degradation requires that substrate proteins contain peptide sequences biochemically related to Lys-Phe-Glu-Arg-Gln (KFERQ).
Related Papers (5)
Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae.
Miki Tsukada,Yoshinori Ohsumi +1 more