Yasuyoshi Sakai

TL;DR: These guidelines are presented for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: A set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes are presented.

TL;DR: The authors would like to thank Drs.W.K. van der Klei, Beth Levine, Fulvio Reggiori, and Takahiro Shintani for helpful comments on the manuscript, and the many researchers in the yeast field who have agreed to changes in the standard names of various genes.

TL;DR: In this article, the authors present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes.

TL;DR: Transient transfection assays using C3H10T1/2 cells suggest that there are several positive regulatory regions in the HES-1 gene, however, cotransfection of the H ES-1 expression vector leads to approximately 40-fold repression in promoter activity, which raises the possibility that Hes1 gene expression may be negatively autoregulated through the N box sequences.