Journal ArticleDOI
Lipid changes of goat sperm plasma membrane during epididymal maturation
TLDR
The altered lipid profile of the mature sperm membrane leads to changes in its fluidity that play an important role in determining the structure and functions of the biomembrane.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1991-01-30. It has received 76 citations till now. The article focuses on the topics: Sperm plasma membrane & Membrane lipids.read more
Citations
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Journal ArticleDOI
Targeted disruption of hormone-sensitive lipase results in male sterility and adipocyte hypertrophy, but not in obesity
Jun-ichi Osuga,Shun Ishibashi,Teruaki Oka,Hiroaki Yagyu,Ryu-ichi Tozawa,Akihisa Fujimoto,Futoshi Shionoiri,Naoya Yahagi,Frederic B. Kraemer,Osamu Tsutsumi,Nobuhiro Yamada +10 more
TL;DR: It is shown that HSL is required for spermatogenesis but is not the only enzyme that mediates the hydrolysis of triacylglycerol stored in adipocytes, and that adipocytes were significantly enlarged and the size differences between cell and tissue suggests the heterogeneity of adipocytes.
Journal ArticleDOI
Bicarbonate stimulated phospholipid scrambling induces cholesterol redistribution and enables cholesterol depletion in the sperm plasma membrane
Frits M. Flesch,Jos F. Brouwers,Patricia F. E. M. Nievelstein,Arie J. Verkleij,Lambert M.G. van Golde,Ben Colenbrander,Barend M. Gadella +6 more
TL;DR: A model is proposed in which phospholipid scrambling induces the formation of an apical membrane raft in the sperm head surface that enables albumin mediated efflux of cholesterol.
Journal ArticleDOI
A porcine homolog of the major secretory protein of human epididymis, HE1, specifically binds cholesterol.
Naomichi Okamura,Sachiko Kiuchi,Michiko Tamba,Takayuki Kashima,Shinsuke Hiramoto,Tadashi Baba,Françoise Dacheux,Jean-Louis Dacheux,Yoshiki Sugita,Yin-Zhe Jin +9 more
TL;DR: It was found that the He1 homolog is a major cholesterol-binding protein in the porcine epididymal fluid and the possibility that the HE1 homology is involved in the regulation of the lipid composition of the sperm membranes during the maturation in epidIDymis is discussed.
Journal ArticleDOI
The epididymis and sperm maturation: a perspective.
TL;DR: In common mammals, sperm leaving the testis are incapable of fertilizing a female gamete, and when assessing sperm maturation it is necessary to establish the proportion of sperm that has completed and retained all steps of maturation necessary to achieve fertilization of oocytes under the conditions imposed.
References
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Journal ArticleDOI
Ficoll gradient isolation of immature sperm of high purity and intactness from goat epididymis.
TL;DR: An improved Ficoll density gradient centrifugation method has been described for the isolation of goat caput-epididymal immature spermatozoa of a high purity and intactness.
Journal ArticleDOI
Lipids and fatty acids of the horseshoe crabsTachypleus gigas andCarcinoscorpius rotundicauda
Satyesh C. Pakrashi,Pradeep K. Duffa,Basudeb Achari,Suniti Misra,A. Choudhury,Subhashis Chattopadhyay,Amitabha Ghosh +6 more
TL;DR: The total lipids from hepatopancreas of the horseshoe crabs, Tachypleus gigas and Carcinus rotundicauda, obtained in 7.6 and 3.3% wet weight respective yields, were fractionated by various chromatographic techniques and identified by gas-liquid chromatography and spectroscopic methods as discussed by the authors.
Journal ArticleDOI
Fatty acids of the mud skipper boleophthalmus boddaerti
TL;DR: The fatty acid composition of the body and the liver lipids of Boleophthalmus boddaerti, a mud skipper fish belonging to the Gobiidae family, have been determined by argentation column chromatography and gas-liquid chromatography.
Journal ArticleDOI
A two-phase polymer method for isolation of maturing goat sperm plasma membrane.
TL;DR: A modified method has been developed by manipulating the centrifugal force (required for membrane sedimentation) for the isolation of maturing sperm plasma membrane of high yield and purity as judged by marker enzyme assays and phase contrast and electron microscopic analyses.
Journal ArticleDOI
Fatty Acids of Thalictrum revolutum Fruit Lipid
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