Journal ArticleDOI
Lipid changes of goat sperm plasma membrane during epididymal maturation
TLDR
The altered lipid profile of the mature sperm membrane leads to changes in its fluidity that play an important role in determining the structure and functions of the biomembrane.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1991-01-30. It has received 76 citations till now. The article focuses on the topics: Sperm plasma membrane & Membrane lipids.read more
Citations
More filters
Journal ArticleDOI
Molecular speciation of fish sperm phospholipids: Large amounts of dipolyunsaturated phosphatidylserine
TL;DR: The molecular species compositions of the main diacyl phosphoglyceride classes and ether-linked subclasses from sperm of three species of fish, sea bass Dicentrarchus labrax, Atlantic salmon Salmo salar and Chinook salmon Onchorhynchus tsawytscha, were determined.
Journal ArticleDOI
Laurdan fluorescence spectroscopy reveals a single liquid-crystalline lipid phase and lack of thermotropic phase transitions in the plasma membrane of living human sperm.
TL;DR: Generalized polarization values derived from Laurdan excitation and emission spectra confirm that the sperm plasma membrane is a low polar, highly rigid (liquid-ordered) structure, and give evidence that, in the range from 10 degrees C to 42 degrees C, membrane lipids are in a single liquid-crystalline phase.
Journal ArticleDOI
Current concepts of molecular events during bovine and porcine spermatozoa capacitation.
TL;DR: The process of sperm capacitation will encompass maturational events that occur following ejaculation up to binding to the zona pellucida, that triggers acrosomal exocytosis and initiates fertilization.
Journal ArticleDOI
Lipid diffusion in the plasma membrane of mouse spermatozoa: changes during epididymal maturation, effects of pH, osmotic pressure, and knockout of the c-ros gene.
TL;DR: Temperature and compositional changes are 2 of the important factors that regulate the dynamics of lipid molecules in the mouse sperm plasma membrane.
Journal ArticleDOI
Expression, Activity, and Subcellular Localization of Testicular Hormone-Sensitive Lipase During Postnatal Development in the Guinea Pig
TL;DR: Insight is contributed regarding the impact of HSL on FC:EC cholesterol ratios and TG levels in the interstitial tissue and tubules in relation to serum testosterone levels during postnatal development, and regarding the immunolocalization of the enzyme in regions of the male gamete consistent with spermatozoa-oocyte interaction.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
Journal ArticleDOI
Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots
TL;DR: Separation of polar lipids by two-dimensional thin layer chromatography providing resolution of all the lipid classes commonly encountered in animal cells and a sensitive, rapid, reproducible procedure for determination of phospholipids by phosphorus analysis of spots are described.
Related Papers (5)
Development changes occurring in the lipids of ram epididymal spermatozoa plasma membrane.
John E. Parks,Roy H. Hammerstedt +1 more
Lipid composition and thermotropic phase behavior of boar, bull, stallion, and rooster sperm membranes.
John E. Parks,Daniel V. Lynch +1 more