Manipulating galectin expression in zebrafish (Danio rerio).
Chiguang Feng,Mihai Nita-Lazar,Nuria González-Montalbán,Jingyu Wang,Justin Mancini,Chinnarajan Ravindran,Chinnarajan Ravindran,Hafiz Ahmed,Gerardo R. Vasta +8 more
TLDR
A review of the current methods available for the manipulation of gene expression in the zebrafish, with a focus on gene knockdown [morpholino (MO)-derived antisense oligonucleotides] and knockout (CRISPR-Cas) technologies.Abstract:
Techniques for disrupting gene expression are invaluable tools for the analysis of the biological role(s) of a gene product. Because of its genetic tractability and multiple advantages over conventional mammalian models, the zebrafish (Danio rerio) is recognized as a powerful system for gaining new insight into diverse aspects of human health and disease. Among the multiple mammalian gene families for which the zebrafish has shown promise as an invaluable model for functional studies, the galectins have attracted great interest due to their participation in early development, regulation of immune homeostasis, and recognition of microbial pathogens. Galectins are β-galactosyl-binding lectins with a characteristic sequence motif in their carbohydrate recognition domains (CRDs), which comprise an evolutionary conserved family ubiquitous in eukaryotic taxa. Galectins are emerging as key players in the modulation of many important pathological processes, which include acute and chronic inflammatory diseases, autoimmunity and cancer, thus making them potential molecular targets for innovative drug discovery. Here, we provide a review of the current methods available for the manipulation of gene expression in the zebrafish, with a focus on gene knockdown [morpholino (MO)-derived antisense oligonucleotides] and knockout (CRISPR-Cas) technologies.read more
Citations
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The zebrafish galectins Drgal1-L2 and Drgal3-L1 bind in vitro to the infectious hematopoietic necrosis virus (IHNV) glycoprotein and reduce viral adhesion to fish epithelial cells
Mihai Nita-Lazar,Justin Mancini,Chiguang Feng,Nuria González-Montalbán,Chinnarajan Ravindran,Shawn Jackson,Ana de las Heras-Sánchez,Barbara Giomarelli,Hafiz Ahmed,Stuart M. Haslam,Gang Wu,Anne Dell,Arun Ammayappan,Vikram N. Vakharia,Gerardo R. Vasta +14 more
TL;DR: It is suggested that the extracellular Drgal1-L2 and Drgal3-L1 interact directly and in a carbohydrate-dependent manner with the IHNV glycosylated envelope and glycans on the epithelial cell surface, significantly reducing viral adhesion.
Journal ArticleDOI
Functions of galectins as 'self/non-self'-recognition and effector factors.
Gerardo R. Vasta,Chiguang Feng,Nuria González-Montalbán,Justin Mancini,Lishi Yang,Kelsey Abernathy,Graeme Frost,Cheyenne Palm +7 more
TL;DR: The structural basis of carbohydrate recognition by galectins, an evolutionary conserved family of ß‐galactoside‐binding lectins, and their binding and effector properties for endogenous (‘self’) and exogenous (“non‐self”) glycosylated ligands are discussed.
Journal ArticleDOI
Pregnancy Galectinology: Insights Into a Complex Network of Glycan Binding Proteins
Sandra M. Blois,Sandra M. Blois,Gabriela S. Dveksler,Gerardo R. Vasta,Nancy Freitag,Véronique Blanchard,Gabriela Barrientos +6 more
TL;DR: The relevance of galectin-glycan interactions as potential therapeutic targets in pregnancy disorders is discussed and the importance of angiogenesis during decidualization and in placenta formation is discussed.
Journal ArticleDOI
Effects of CRISPR/Cas9 dosage on TICAM1 and RBL gene mutation rate, embryonic development, hatchability and fry survival in channel catfish
Ahmed Elaswad,Karim Khalil,Karim Khalil,Zhi Ye,Zhanjiang Liu,Zhanjiang Liu,Shikai Liu,Shikai Liu,Eric Peatman,Ramjie Odin,Ramjie Odin,Khoi Vo,David Drescher,David Drescher,Kamal Gosh,Kamal Gosh,Guyu Qin,William Bugg,William Bugg,Nathan J. C. Backenstose,Nathan J. C. Backenstose,Rex A. Dunham +21 more
TL;DR: The current results lay the foundations for designing gene editing experiments in channel catfish and can be used as a guide for other fish species.
Journal ArticleDOI
Galectin-mediated immune recognition: Opsonic roles with contrasting outcomes in selected shrimp and bivalve mollusk species.
Gerardo R. Vasta,Jin-Xing Wang +1 more
TL;DR: The results suggest that although galectins from bivalves can function as PRRs, Perkinsus parasites have co-evolved with their hosts to subvert the galectin' immune functions for host infection by acquisition of carbohydrate-based mimicry.
References
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Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,F. A. Ran,David Benjamin Turitz Cox,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +10 more
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Journal ArticleDOI
Efficient genome editing in zebrafish using a CRISPR-Cas system
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TL;DR: It is shown that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.