Journal ArticleDOI
Nanopore analytics: sensing of single molecules
Stefan Howorka,Zuzanna S. Siwy +1 more
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TLDR
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current, which ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules.Abstract:
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current. Reflecting its simplicity, nanopore analytics has gained popularity and can be conducted with natural protein as well as man-made polymeric and inorganic pores. The spectrum of detectable analytes ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules. Apart from being an analytical tool, nanopores have developed into a general platform technology to investigate the biophysics, physicochemistry, and chemistry of individual molecules (critical review, 310 references).read more
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Journal ArticleDOI
Nanoscale Investigation of Generation 1 PAMAM Dendrimers Interaction with a Protein Nanopore.
Alina Asandei,Andrei Ciuca,Aurelia Apetrei,Irina Schiopu,Loredana Mereuta,Chang Ho Seo,Yoonkyung Park,Tudor Luchian +7 more
TL;DR: It is shown that the ability of dendrimer molecules to adapt their structure to nanoscopic spaces, and control the flow of matter through the α-hemolysin nanopore, depends non-trivially on the pH- and salt-induced conformational changes of the dendedrimer.
Journal ArticleDOI
N-Terminal Derivatization-Assisted Identification of Individual Amino Acids Using a Biological Nanopore Sensor.
Xiaojun Wei,Dumei Ma,Dumei Ma,Zehui Zhang,Leon Y Wang,Jonathan L Gray,Libo Zhang,Tianyu Zhu,Xiaoqin Wang,Brian Lenhart,Yingwu Yin,Qian Wang,Chang Liu +12 more
TL;DR: The feasibility of derivatization-assisted identification of AAs regardless of their charge composition and polarity is demonstrated and will not only pave a way for identification of individual AAs but also lead to future applications in protein/peptide sequencing using the nanopore technology.
Journal ArticleDOI
Magnetic microbead transport during resistive pulse sensing
TL;DR: Tunable resistive pulse sensing experiments have been used to quantitatively study the motion of 1 μm superparamagnetic beads in a variable magnetic field, and closed-form theory has been developed to interpret the experiments, incorporating six particle transport mechanisms which depend on particle position in and near a conical pore.
Journal ArticleDOI
Membrane platforms for biological nanopore sensing and sequencing.
TL;DR: In the past two decades, biological nanopores have been developed and explored for use in sensing applications as a result of their exquisite sensitivity and easily engineered, reproducible, and economically manufactured structures as mentioned in this paper.
Journal ArticleDOI
Rational Design of Stimuli-Responsive Polymers Modified Nanopores for Selective and Sensitive Determination of Salivary Glucose
TL;DR: A new platform for glucose detection in saliva is unveiled, and a new strategy for detecting other biomolecules in accessible biofluid involved in physiological and pathological events is promised.
References
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Journal ArticleDOI
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
TL;DR: The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches.
Journal ArticleDOI
Formation and Structure of Self-Assembled Monolayers.
TL;DR: Monolayers of alkanethiolates on gold are probably the most studied SAMs to date and offer the needed design flexibility, both at the individual molecular and at the material levels, and offer a vehicle for investigation of specific interactions at interfaces, and of the effect of increasing molecular complexity on the structure and stability of two-dimensional assemblies.
Journal ArticleDOI
Characterization of individual polynucleotide molecules using a membrane channel
TL;DR: It is shown that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane, which could in principle provide direct, high-speed detection of the sequence of bases in single molecules of DNA or RNA.
Book ChapterDOI
Ion Channels in Excitable Membranes
TL;DR: The action potential is triggered when the membrane potential, which was at the resting level, depolarizes and reaches the threshold of excitation, which triggers the action potential.
Journal ArticleDOI
The Potential and Challenges of Nanopore Sequencing
Daniel Branton,David W. Deamer,Andre Marziali,Hagan Bayley,Steven A. Benner,Thomas Z. Butler,Massimiliano Di Ventra,Slaven Garaj,Andrew Hibbs,Xiaohua Huang,Stevan B Jovanovich,Predrag S Krstic,Stuart Lindsay,Xinsheng Sean Ling,Carlos H. Mastrangelo,Amit Meller,John S. Oliver,Yuriy V. Pershin,J. Michael Ramsey,Robert Riehn,Gautam V. Soni,Vincent Tabard-Cossa,Meni Wanunu,Matthew Wiggin,Jeffery A. Schloss +24 more
TL;DR: A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility.
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