Journal ArticleDOI
Nanopore analytics: sensing of single molecules
Stefan Howorka,Zuzanna S. Siwy +1 more
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TLDR
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current, which ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules.Abstract:
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current. Reflecting its simplicity, nanopore analytics has gained popularity and can be conducted with natural protein as well as man-made polymeric and inorganic pores. The spectrum of detectable analytes ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules. Apart from being an analytical tool, nanopores have developed into a general platform technology to investigate the biophysics, physicochemistry, and chemistry of individual molecules (critical review, 310 references).read more
Citations
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Journal ArticleDOI
Design and fabrication of a biomimetic nanochannel for highly sensitive arginine response in serum samples.
TL;DR: A aldehyde calix[4]arene (C4AH) is designed and prepared, which was attached to the interior surface of a single nanochannel by using a click reaction, and that showed a high response for arginine (Arg).
Journal ArticleDOI
Scalable fabrication of sub-10 nm polymer nanopores for DNA analysis
TL;DR: This new approach enables researchers to produce with high throughput nanopore devices with precisely positioned sub-10 nm pores which can be used to detect and analyze individual biomolecules.
Journal ArticleDOI
Ion exclusion and electrokinetic effects resulting from electro-osmotic flow of salt solutions in charged silica nanopores.
TL;DR: In this paper, the results of extensive all-atom molecular dynamics simulations of the electro-osmotic flow of 0.5 M monovalent (NaCl) and divalent (CaCl(2)) ionic solutions through cylindrical charged silica nanopores are presented.
Journal ArticleDOI
Structural Destabilization of DNA Duplexes Containing Single-Base Lesions Investigated by Nanopore Measurements
TL;DR: A large distortion of the duplex backbone introduced by the presence of the highly oxidized guanine products Sp and Gh leads to a rapid two-step unzipping, and the number of hydrogen bonds in the modified base pair plays a lesser role in determining the kinetics of duplex dissociation.
Journal ArticleDOI
A biosensing expedition of nanopore: A review
TL;DR: A review of nanopore-assisted, nanoporebased detection over different types of porous matrices, including biological, solid-state, two-dimensional metal-organic framework, and hybrid matrices along with their detailed descriptions, keeping present and future perspectives in mind is provided in this article.
References
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Journal ArticleDOI
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
TL;DR: The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches.
Journal ArticleDOI
Formation and Structure of Self-Assembled Monolayers.
TL;DR: Monolayers of alkanethiolates on gold are probably the most studied SAMs to date and offer the needed design flexibility, both at the individual molecular and at the material levels, and offer a vehicle for investigation of specific interactions at interfaces, and of the effect of increasing molecular complexity on the structure and stability of two-dimensional assemblies.
Journal ArticleDOI
Characterization of individual polynucleotide molecules using a membrane channel
TL;DR: It is shown that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane, which could in principle provide direct, high-speed detection of the sequence of bases in single molecules of DNA or RNA.
Book ChapterDOI
Ion Channels in Excitable Membranes
TL;DR: The action potential is triggered when the membrane potential, which was at the resting level, depolarizes and reaches the threshold of excitation, which triggers the action potential.
Journal ArticleDOI
The Potential and Challenges of Nanopore Sequencing
Daniel Branton,David W. Deamer,Andre Marziali,Hagan Bayley,Steven A. Benner,Thomas Z. Butler,Massimiliano Di Ventra,Slaven Garaj,Andrew Hibbs,Xiaohua Huang,Stevan B Jovanovich,Predrag S Krstic,Stuart Lindsay,Xinsheng Sean Ling,Carlos H. Mastrangelo,Amit Meller,John S. Oliver,Yuriy V. Pershin,J. Michael Ramsey,Robert Riehn,Gautam V. Soni,Vincent Tabard-Cossa,Meni Wanunu,Matthew Wiggin,Jeffery A. Schloss +24 more
TL;DR: A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility.
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