Journal ArticleDOI
Nanopore analytics: sensing of single molecules
Stefan Howorka,Zuzanna S. Siwy +1 more
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TLDR
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current, which ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules.Abstract:
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current. Reflecting its simplicity, nanopore analytics has gained popularity and can be conducted with natural protein as well as man-made polymeric and inorganic pores. The spectrum of detectable analytes ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules. Apart from being an analytical tool, nanopores have developed into a general platform technology to investigate the biophysics, physicochemistry, and chemistry of individual molecules (critical review, 310 references).read more
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Journal ArticleDOI
A New Cells‐Compatible Microfluidic Device for Single Channel Recordings
Manuela Zanetti,Devid Maniglio,Cristian Fasoli,Marco Pola,Elisa Borga,Claudio Corradi,Mauro Dalla Serra,Salvatore Iannotta,Antonella Motta,Tullio Toccoli +9 more
TL;DR: The fabrication of a microfluidic apparatus and the realization of a sensors based on PEDOT, a biocompatible semiconductor polymer used in substitution of standard electrodes for electrophysiological studies and for detection of nanopores in membrane, gives the possibility to study the mechanisms of ions balance and molecular transport though cell membranes.
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Sensing with nanopores – the influence of asymmetric blocking on electrochemical redox cycling current
Kay J. Krause,Enno Kätelhön,Serge G. Lemay,Richard G. Compton,Bernhard Wolfrum,Bernhard Wolfrum +5 more
TL;DR: This work investigates the influence of different blocking scenarios inside a nanopore on the resulting redox cycling current and distinguishes between symmetric and asymmetric pore blocking and shows that the current decrease is more pronounced in the case of asymmetric blocking reflecting the diffusion-driven pathway of the redox-active molecules.
Journal ArticleDOI
Improved manufacture of hybrid membranes with bionanopore adapters capable of self-luting
Klara Altintoprak,Farid Farajollahi,Axel Seidenstücker,Timo Ullrich,Nana L. Wenz,Peter Krolla,Alfred Plettl,Paul J. Ziemann,Othmar Marti,Paul Walther,Daniela Exner,Ruth Schwaiger,Hartmut Gliemann,Christina Wege +13 more
TL;DR: The authors’ recent concept of bioinorganic filtration devices made up of solid-state membranes (SSMs) accommodating ring-shaped, ribonucleic acid (RNA)-stabilized tobacco mosaic virus (TMV) coat p...
Journal ArticleDOI
Membrane-Suspended Nanopores in Microchip Arrays for Stochastic Transport Recording and Sensing
Tim Diederichs,Robert Tampé +1 more
TL;DR: In this article, the authors summarize recent developments and compare features of membrane embedded nanopores with solid state analogues, and describe how their translocation properties are characterized by microchip systems.
References
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Journal ArticleDOI
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
TL;DR: The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches.
Journal ArticleDOI
Formation and Structure of Self-Assembled Monolayers.
TL;DR: Monolayers of alkanethiolates on gold are probably the most studied SAMs to date and offer the needed design flexibility, both at the individual molecular and at the material levels, and offer a vehicle for investigation of specific interactions at interfaces, and of the effect of increasing molecular complexity on the structure and stability of two-dimensional assemblies.
Journal ArticleDOI
Characterization of individual polynucleotide molecules using a membrane channel
TL;DR: It is shown that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane, which could in principle provide direct, high-speed detection of the sequence of bases in single molecules of DNA or RNA.
Book ChapterDOI
Ion Channels in Excitable Membranes
TL;DR: The action potential is triggered when the membrane potential, which was at the resting level, depolarizes and reaches the threshold of excitation, which triggers the action potential.
Journal ArticleDOI
The Potential and Challenges of Nanopore Sequencing
Daniel Branton,David W. Deamer,Andre Marziali,Hagan Bayley,Steven A. Benner,Thomas Z. Butler,Massimiliano Di Ventra,Slaven Garaj,Andrew Hibbs,Xiaohua Huang,Stevan B Jovanovich,Predrag S Krstic,Stuart Lindsay,Xinsheng Sean Ling,Carlos H. Mastrangelo,Amit Meller,John S. Oliver,Yuriy V. Pershin,J. Michael Ramsey,Robert Riehn,Gautam V. Soni,Vincent Tabard-Cossa,Meni Wanunu,Matthew Wiggin,Jeffery A. Schloss +24 more
TL;DR: A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility.
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The Potential and Challenges of Nanopore Sequencing
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