The Potential and Challenges of Nanopore Sequencing
Daniel Branton,David W. Deamer,Andre Marziali,Hagan Bayley,Steven A. Benner,Thomas Z. Butler,Massimiliano Di Ventra,Slaven Garaj,Andrew Hibbs,Xiaohua Huang,Stevan B Jovanovich,Predrag S Krstic,Stuart Lindsay,Xinsheng Sean Ling,Carlos H. Mastrangelo,Amit Meller,John S. Oliver,Yuriy V. Pershin,J. Michael Ramsey,Robert Riehn,Gautam V. Soni,Vincent Tabard-Cossa,Meni Wanunu,Matthew Wiggin,Jeffery A. Schloss +24 more
TLDR
A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility.Abstract:
A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore. The nanopore provides a highly confined space within which single nucleic acid polymers can be analyzed at high throughput by one of a variety of means, and the perfect processivity that can be enforced in a narrow pore ensures that the native order of the nucleobases in a polynucleotide is reflected in the sequence of signals that is detected. Kilobase length polymers (single-stranded genomic DNA or RNA) or small molecules (e.g., nucleosides) can be identified and characterized without amplification or labeling, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility. Further research and development to overcome current challenges to nanopore identification of each successive nucleotide in a DNA strand offers the prospect of 'third generation' instruments that will sequence a diploid mammalian genome for ∼$1,000 in ∼24 h.read more
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