Journal ArticleDOI
Nanopore analytics: sensing of single molecules
Stefan Howorka,Zuzanna S. Siwy +1 more
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TLDR
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current, which ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules.Abstract:
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current. Reflecting its simplicity, nanopore analytics has gained popularity and can be conducted with natural protein as well as man-made polymeric and inorganic pores. The spectrum of detectable analytes ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules. Apart from being an analytical tool, nanopores have developed into a general platform technology to investigate the biophysics, physicochemistry, and chemistry of individual molecules (critical review, 310 references).read more
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Journal ArticleDOI
Differentiation of short, single-stranded DNA homopolymers in solid-state nanopores.
Kimberly Venta,Gabriel Shemer,Matthew Puster,Julio A. Rodríguez-Manzo,Adrian Balan,Jacob K. Rosenstein,Jacob K. Rosenstein,Kenneth L. Shepard,Marija Drndic +8 more
TL;DR: It is shown that small silicon nitride nanopores can resolve differences between ionic current signals produced by short ssDNA homopolymers, when combined with measurement electronics that allow a signal-to-noise ratio of better than 10 to be achieved at 1-MHz bandwidth.
Journal ArticleDOI
Building Bio-Inspired Artificial Functional Nanochannels: From Symmetric to Asymmetric Modification
Xu Hou,Huacheng Zhang,Lei Jiang +2 more
TL;DR: This Minireview summary of recent developments in building functional nanochannels by applying various symmetric and asymmetric modifications is summarized.
Journal ArticleDOI
Building membrane nanopores
TL;DR: This Review critically compares the characteristics of the different building materials, and explores the influence of the building material on pore structure, dynamics and function.
Journal ArticleDOI
Size Dependent Surface Charge Properties of Silica Nanoparticles
TL;DR: In this paper, surface charge properties of silica nanoparticles with different sizes were studied using a multi-ion surface charge-regulation model, where protonation and deprotonation surface reactions were used to obtain the local surface charge, which depends on the particle size and electrolyte solution properties, including salt concentration and pH.
Journal ArticleDOI
Enantioselective Recognition in Biomimetic Single Artificial Nanochannels
TL;DR: A simple enantioselective sensing device based on a single artificial β-cyclodextrin-modified nanochannel system shows highly selective recognition of histidine enantiomers through monitoring of ionic current signatures.
References
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Journal ArticleDOI
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
TL;DR: The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches.
Journal ArticleDOI
Formation and Structure of Self-Assembled Monolayers.
TL;DR: Monolayers of alkanethiolates on gold are probably the most studied SAMs to date and offer the needed design flexibility, both at the individual molecular and at the material levels, and offer a vehicle for investigation of specific interactions at interfaces, and of the effect of increasing molecular complexity on the structure and stability of two-dimensional assemblies.
Journal ArticleDOI
Characterization of individual polynucleotide molecules using a membrane channel
TL;DR: It is shown that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane, which could in principle provide direct, high-speed detection of the sequence of bases in single molecules of DNA or RNA.
Book ChapterDOI
Ion Channels in Excitable Membranes
TL;DR: The action potential is triggered when the membrane potential, which was at the resting level, depolarizes and reaches the threshold of excitation, which triggers the action potential.
Journal ArticleDOI
The Potential and Challenges of Nanopore Sequencing
Daniel Branton,David W. Deamer,Andre Marziali,Hagan Bayley,Steven A. Benner,Thomas Z. Butler,Massimiliano Di Ventra,Slaven Garaj,Andrew Hibbs,Xiaohua Huang,Stevan B Jovanovich,Predrag S Krstic,Stuart Lindsay,Xinsheng Sean Ling,Carlos H. Mastrangelo,Amit Meller,John S. Oliver,Yuriy V. Pershin,J. Michael Ramsey,Robert Riehn,Gautam V. Soni,Vincent Tabard-Cossa,Meni Wanunu,Matthew Wiggin,Jeffery A. Schloss +24 more
TL;DR: A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility.
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