Journal ArticleDOI
Nanopore analytics: sensing of single molecules
Stefan Howorka,Zuzanna S. Siwy +1 more
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TLDR
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current, which ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules.Abstract:
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current. Reflecting its simplicity, nanopore analytics has gained popularity and can be conducted with natural protein as well as man-made polymeric and inorganic pores. The spectrum of detectable analytes ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules. Apart from being an analytical tool, nanopores have developed into a general platform technology to investigate the biophysics, physicochemistry, and chemistry of individual molecules (critical review, 310 references).read more
Citations
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Journal ArticleDOI
Superposition of an AC field improves the discrimination between peptides in nanopore analysis
Elisabet Jakova,Jeremy S. Lee +1 more
TL;DR: It was shown that the ratio of translocations to bumping events could be manipulated by a combination of AC voltages and frequencies and large, intrinsically disordered protein, α-synuclein, which translocates the pore readily in a DC field could be prevented from doing so by application of an AC field of 200 mV at 100 MHz.
Book ChapterDOI
Chapter 2 – DNA Translocation
Oliver Otto,Ulrich F. Keyser +1 more
TL;DR: The concepts of electrostatic screening in ionic solutions are introduced and the relevant physical processes of driven DNA translocation DNA are shown focusing on the interplay between electroosmosis, electrophoresis, and hydrodynamic interactions.
Proceedings ArticleDOI
Graphene Field Effect Nanopore Glycine Detector
Feras Al-Dirini,Sharafat Hossain,Wanzhi Qiu,Faruque M. Hossain,Ampalavanapillai Nirmalathas,Efstratios Skafidas +5 more
TL;DR: In this article, a new class of Graphene Nanopores that are tunable by means of a lateral in-plane field effect is presented, which is self-induced and does not require an additional gate terminal, and results in strong control over the channel's conductivity.
Journal ArticleDOI
Panorama of the Intracellular Molecular Concert Orchestrated by Actinoporins, Pore-Forming Toxins from Sea Anemones.
Carlos Alvarez,Carmen Soto,Sheila Cabezas,Javier Alvarado-Mesén,Rady J. Laborde,Fabiola Pazos,Uris Ros,Ana María Hernández,María E. Lanio +8 more
TL;DR: In this article, an updated landscape of the studies aimed at understanding the intracellular pathways triggered in response to sticholysin II, the most active isoform produced by the Caribbean Sea anemone Stichodactyla helianthus, is presented and discussed.
Journal ArticleDOI
Review of the use of nanodevices to detect single molecules.
TL;DR: The development of methodologies to identify single molecules and/or to detect/monitor molecular behavior at the single-molecule level is one of the important research topics in chemistry and biology as discussed by the authors .
References
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Journal ArticleDOI
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
TL;DR: The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches.
Journal ArticleDOI
Formation and Structure of Self-Assembled Monolayers.
TL;DR: Monolayers of alkanethiolates on gold are probably the most studied SAMs to date and offer the needed design flexibility, both at the individual molecular and at the material levels, and offer a vehicle for investigation of specific interactions at interfaces, and of the effect of increasing molecular complexity on the structure and stability of two-dimensional assemblies.
Journal ArticleDOI
Characterization of individual polynucleotide molecules using a membrane channel
TL;DR: It is shown that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane, which could in principle provide direct, high-speed detection of the sequence of bases in single molecules of DNA or RNA.
Book ChapterDOI
Ion Channels in Excitable Membranes
TL;DR: The action potential is triggered when the membrane potential, which was at the resting level, depolarizes and reaches the threshold of excitation, which triggers the action potential.
Journal ArticleDOI
The Potential and Challenges of Nanopore Sequencing
Daniel Branton,David W. Deamer,Andre Marziali,Hagan Bayley,Steven A. Benner,Thomas Z. Butler,Massimiliano Di Ventra,Slaven Garaj,Andrew Hibbs,Xiaohua Huang,Stevan B Jovanovich,Predrag S Krstic,Stuart Lindsay,Xinsheng Sean Ling,Carlos H. Mastrangelo,Amit Meller,John S. Oliver,Yuriy V. Pershin,J. Michael Ramsey,Robert Riehn,Gautam V. Soni,Vincent Tabard-Cossa,Meni Wanunu,Matthew Wiggin,Jeffery A. Schloss +24 more
TL;DR: A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility.
Related Papers (5)
The Potential and Challenges of Nanopore Sequencing
Daniel Branton,David W. Deamer,Andre Marziali,Hagan Bayley,Steven A. Benner,Thomas Z. Butler,Massimiliano Di Ventra,Slaven Garaj,Andrew Hibbs,Xiaohua Huang,Stevan B Jovanovich,Predrag S Krstic,Stuart Lindsay,Xinsheng Sean Ling,Carlos H. Mastrangelo,Amit Meller,John S. Oliver,Yuriy V. Pershin,J. Michael Ramsey,Robert Riehn,Gautam V. Soni,Vincent Tabard-Cossa,Meni Wanunu,Matthew Wiggin,Jeffery A. Schloss +24 more