Journal ArticleDOI
Nanopore analytics: sensing of single molecules
Stefan Howorka,Zuzanna S. Siwy +1 more
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TLDR
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current, which ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules.Abstract:
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current. Reflecting its simplicity, nanopore analytics has gained popularity and can be conducted with natural protein as well as man-made polymeric and inorganic pores. The spectrum of detectable analytes ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules. Apart from being an analytical tool, nanopores have developed into a general platform technology to investigate the biophysics, physicochemistry, and chemistry of individual molecules (critical review, 310 references).read more
Citations
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Science and technology roadmap for graphene, related two-dimensional crystals, and hybrid systems
Andrea C. Ferrari,Francesco Bonaccorso,Francesco Bonaccorso,Vladimir I. Fal'ko,Konstantin S. Novoselov,Stephan Roche,Peter Bøggild,Stefano Borini,Frank H. L. Koppens,Vincenzo Palermo,Nicola M. Pugno,Nicola M. Pugno,Nicola M. Pugno,Jose A. Garrido,Roman Sordan,Alberto Bianco,Laura Ballerini,Maurizio Prato,Elefterios Lidorikis,Jani Kivioja,Claudio Marinelli,Tapani Ryhänen,Alberto F. Morpurgo,Jonathan N. Coleman,Valeria Nicolosi,Luigi Colombo,Albert Fert,Albert Fert,Mar García-Hernández,Adrian Bachtold,Grégory F. Schneider,Francisco Guinea,Cees Dekker,Matteo Barbone,Zhipei Sun,Costas Galiotis,Alexander N. Grigorenko,Gerasimos Konstantatos,Andras Kis,Mikhail I. Katsnelson,Lieven M. K. Vandersypen,A. Loiseau,Vittorio Morandi,Daniel Neumaier,Emanuele Treossi,Vittorio Pellegrini,Vittorio Pellegrini,Marco Polini,Alessandro Tredicucci,Gareth M. Williams,Byung Hee Hong,Jong Hyun Ahn,Jong Min Kim,Herbert Zirath,Bart J. van Wees,Herre S. J. van der Zant,Luigi Occhipinti,Andrea di Matteo,Ian A. Kinloch,Thomas Seyller,Etienne Quesnel,Xinliang Feng,K.B.K. Teo,Nalin Rupesinghe,Pertti Hakonen,Simon R. T. Neil,Quentin Tannock,Tomas Löfwander,Jari M. Kinaret +68 more
TL;DR: An overview of the key aspects of graphene and related materials, ranging from fundamental research challenges to a variety of applications in a large number of sectors, highlighting the steps necessary to take GRMs from a state of raw potential to a point where they might revolutionize multiple industries are provided.
Journal ArticleDOI
Rapid electronic detection of probe-specific microRNAs using thin nanopore sensors
TL;DR: A platform for the rapid electronic detection of probe-hybridized microRNAs from cellular RNA is presented, in which a target microRNA is first hybridized to a probe and enriched through binding to the viral protein p19.
Journal ArticleDOI
Synthetic Lipid Membrane Channels Formed by Designed DNA Nanostructures
Martin Langecker,Vera Arnaut,Thomas G. Martin,Jonathan List,Stephan Renner,Michael Mayer,Hendrik Dietz,Friedrich C. Simmel +7 more
TL;DR: In single-channel electrophysiological measurements, the authors found similarities to the response of natural ion channels, such as conductances on the order of 1 nanosiemens and channel gating, and show that the synthetic channels can be used to discriminate single DNA molecules.
Journal ArticleDOI
Nanopores: A journey towards DNA sequencing
TL;DR: Past and current studies related to nucleic acid biophysics are surveyed, and will hopefully provoke a discussion of immediate and future prospects for the field.
Journal ArticleDOI
Controlling protein translocation through nanopores with bio-inspired fluid walls
Erik Yusko,Jay M. Johnson,Sheereen Majd,Panchika Prangkio,Ryan Rollings,Jiali Li,Jerry Yang,Michael Mayer +7 more
TL;DR: It is shown that coating nanopores with fluid bilayer lipids allows the pore diameters to be fine-tuned in sub-nanometre increments, and incorporation of mobile ligands in the lipid conferred specificity and slowed down the translocation of targeted proteins sufficiently to time-resolve translocation events of individual proteins.
References
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Journal ArticleDOI
Ion exclusion by sub-2-nm carbon nanotube pores
Francesco Fornasiero,Hyung Gyu Park,Jason K. Holt,Michael Stadermann,Costas P. Grigoropoulos,Aleksandr Noy,Aleksandr Noy,Olgica Bakajin,Olgica Bakajin +8 more
TL;DR: It is shown that carbon nanotube membranes exhibit significant ion exclusion that can be as high as 98% under certain conditions, which strongly support a Donnan-type rejection mechanism, dominated by electrostatic interactions between fixed membrane charges and mobile ions.
Journal ArticleDOI
Metal Nanotubule Membranes with Electrochemically Switchable Ion-Transport Selectivity
TL;DR: Membranes containing cylindrical metal nanotubules that span the complete thickness of the membrane are described, showing selective ion transport analogous to that observed in ion-exchange polymers.
Journal ArticleDOI
Direct force measurements on DNA in a solid-state nanopore
Ulrich F. Keyser,Bernard N. Koeleman,Stijn van Dorp,Diego Krapf,Ralph M. M. Smeets,Serge G. Lemay,Nynke H. Dekker,Cees Dekker +7 more
TL;DR: The first measurements of the force on a single DNA molecule in a solid-state nanopore are demonstrated by combining optical tweezers11 with ionic-current detection and can be used to slow down and even arrest the translocation of the DNA molecules.
Journal ArticleDOI
Molecular determinants of Ca2+ selectivity and ion permeation in L-type Ca2+ channels.
TL;DR: A systematic series of single amino-acid substitutions is carried out and all four glutamates participate in high-affinity binding of Ca2+ or Cdd2+.
Journal ArticleDOI
Nanotubule-Based Molecular-Filtration Membranes
TL;DR: In this article, a simple membrane permeation experiment was performed to cleanly separate small molecules on the basis of molecular size using a collection of monodisperse gold nanotubules with inside diameters of molecular dimensions (less than 1 nanometer).
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