Journal ArticleDOI
Nanopore analytics: sensing of single molecules
Stefan Howorka,Zuzanna S. Siwy +1 more
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TLDR
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current, which ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules.Abstract:
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current. Reflecting its simplicity, nanopore analytics has gained popularity and can be conducted with natural protein as well as man-made polymeric and inorganic pores. The spectrum of detectable analytes ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules. Apart from being an analytical tool, nanopores have developed into a general platform technology to investigate the biophysics, physicochemistry, and chemistry of individual molecules (critical review, 310 references).read more
Citations
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Journal ArticleDOI
Biomedical diagnosis perspective of epigenetic detections using alpha-hemolysin nanopore
Yong Wang,Li-Qun Gu +1 more
TL;DR: Several challenges arise in applying nanopore devices to clinical studies, including super low physiological concentrations of biomarkers resulting in low sensitivity, complex biological sample contents resulting in false signals, and fast translocating speed through the pore resulting in poor detections.
Journal ArticleDOI
Size and functional tuning of solid state nanopores by chemical functionalization.
TL;DR: The presented results show that, by varying the silanization time, it is possible to modify the efficiency of probe molecule attachment, thus shrinking the pore to the chosen size, while introducing a specific sensing selectivity.
Journal ArticleDOI
Smart pH-Modulated Two-Way Photoswitch Based on a Polymer-Modified Single Nanochannel.
TL;DR: In this paper, a pH-modulated two-way photowitch was demonstrated by functionalizing the interior of a single conical glass nanochannel with a poly-spiropyran-linked methacrylate (P-SPMA) polymer through surface-initiated atom transfer radical polymerization.
Journal ArticleDOI
pH-modulated ion transport and amplified redox response of Keggin-type polyoxometalates through vertically-oriented mesoporous silica nanochannels
TL;DR: In this article, a cyclic voltammetry has been applied to characterize mass transport of a Keggin-type polyoxometalates (SiW12O40]4-) through vertically-aligned mesoporous silica thin films in aqueous media.
Journal ArticleDOI
The rectification of mono- and bivalent ions in single conical nanopores
Junzhe Wei,Guanghua Du,Jinlong Guo,Yaning Li,Wenjing Liu,Huijun Yao,Jing Zhao,Ruqun Wu,Hao Chen,Artem Ponomarov +9 more
TL;DR: In this article, the conical nanopores have rectified transportation of these cations at the applied voltage of between +2 V and −2 V, and the rectification coefficient γ of the mono- and bivalent ions was determined in their solution of 0.0001 − 1 M measured at 1 V.
References
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Journal ArticleDOI
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
TL;DR: The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches.
Journal ArticleDOI
Formation and Structure of Self-Assembled Monolayers.
TL;DR: Monolayers of alkanethiolates on gold are probably the most studied SAMs to date and offer the needed design flexibility, both at the individual molecular and at the material levels, and offer a vehicle for investigation of specific interactions at interfaces, and of the effect of increasing molecular complexity on the structure and stability of two-dimensional assemblies.
Journal ArticleDOI
Characterization of individual polynucleotide molecules using a membrane channel
TL;DR: It is shown that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane, which could in principle provide direct, high-speed detection of the sequence of bases in single molecules of DNA or RNA.
Book ChapterDOI
Ion Channels in Excitable Membranes
TL;DR: The action potential is triggered when the membrane potential, which was at the resting level, depolarizes and reaches the threshold of excitation, which triggers the action potential.
Journal ArticleDOI
The Potential and Challenges of Nanopore Sequencing
Daniel Branton,David W. Deamer,Andre Marziali,Hagan Bayley,Steven A. Benner,Thomas Z. Butler,Massimiliano Di Ventra,Slaven Garaj,Andrew Hibbs,Xiaohua Huang,Stevan B Jovanovich,Predrag S Krstic,Stuart Lindsay,Xinsheng Sean Ling,Carlos H. Mastrangelo,Amit Meller,John S. Oliver,Yuriy V. Pershin,J. Michael Ramsey,Robert Riehn,Gautam V. Soni,Vincent Tabard-Cossa,Meni Wanunu,Matthew Wiggin,Jeffery A. Schloss +24 more
TL;DR: A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility.
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The Potential and Challenges of Nanopore Sequencing
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