Journal ArticleDOI
Nanopore analytics: sensing of single molecules
Stefan Howorka,Zuzanna S. Siwy +1 more
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TLDR
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current, which ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules.Abstract:
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current. Reflecting its simplicity, nanopore analytics has gained popularity and can be conducted with natural protein as well as man-made polymeric and inorganic pores. The spectrum of detectable analytes ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules. Apart from being an analytical tool, nanopores have developed into a general platform technology to investigate the biophysics, physicochemistry, and chemistry of individual molecules (critical review, 310 references).read more
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Journal ArticleDOI
Nanopore Detection Assisted DNA Information Processing
Zichen Song,Yuan Liang,Jing Yang +2 more
TL;DR: The principles and development history of nanopore detection are introduced and a systematic review of recent developments and specific applications in DNA information processing involving nanopore Detection and nanopore-based storage are conducted.
Journal ArticleDOI
Solid-state nanopore: chemical modifications, interactions, and functionalities.
TL;DR: In this article , the authors summarize the recent research on nanopore modification to overcome the drawbacks of low signal-to-noise ratio and low resolution, which hinders their practical applications.
Journal ArticleDOI
A single-molecule insight into the ionic strength dependent, cationic peptide nucleic acids - oligonucleotides interactions.
TL;DR: A scenario in which reduced charge screening by counterions in low salt electrolytes enables non-specific, electrostatic interactions with the anionic backbone of polynucleotides, thus reducing the ability of PNA-DNA complementary association via hydrogen bonding patterns is suggested.
Journal ArticleDOI
Polypeptide analysis for nanopore-based protein identification
Mazdak Afshar Bakshloo,Safia Yahiaoui,Fabien Piguet,Manuela Pastoriza-Gallego,Régis Daniel,Jérôme Mathé,John J. Kasianowicz,Abdelghani Oukhaled +7 more
TL;DR: Although additional experiments, computer simulations, and artificial intelligence research are needed to better understand how to optimize the partitioning of enzymatically cleaved fragments into the AeL nanopore, the method is still capable of accurately identifying proteins.
Journal ArticleDOI
Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy.
Avani V. Pisapati,Yi Wang,Megan E. Blauch,Nathan J. Wittenberg,Xuanhong Cheng,X. Frank Zhang +5 more
TL;DR: The reversible unraveling dynamics of VWF are useful for understanding how its function is regulated in human blood, while the conformation of lambda DNA offers insights into the biophysics of macromolecules.
References
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Journal ArticleDOI
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
TL;DR: The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches.
Journal ArticleDOI
Formation and Structure of Self-Assembled Monolayers.
TL;DR: Monolayers of alkanethiolates on gold are probably the most studied SAMs to date and offer the needed design flexibility, both at the individual molecular and at the material levels, and offer a vehicle for investigation of specific interactions at interfaces, and of the effect of increasing molecular complexity on the structure and stability of two-dimensional assemblies.
Journal ArticleDOI
Characterization of individual polynucleotide molecules using a membrane channel
TL;DR: It is shown that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane, which could in principle provide direct, high-speed detection of the sequence of bases in single molecules of DNA or RNA.
Book ChapterDOI
Ion Channels in Excitable Membranes
TL;DR: The action potential is triggered when the membrane potential, which was at the resting level, depolarizes and reaches the threshold of excitation, which triggers the action potential.
Journal ArticleDOI
The Potential and Challenges of Nanopore Sequencing
Daniel Branton,David W. Deamer,Andre Marziali,Hagan Bayley,Steven A. Benner,Thomas Z. Butler,Massimiliano Di Ventra,Slaven Garaj,Andrew Hibbs,Xiaohua Huang,Stevan B Jovanovich,Predrag S Krstic,Stuart Lindsay,Xinsheng Sean Ling,Carlos H. Mastrangelo,Amit Meller,John S. Oliver,Yuriy V. Pershin,J. Michael Ramsey,Robert Riehn,Gautam V. Soni,Vincent Tabard-Cossa,Meni Wanunu,Matthew Wiggin,Jeffery A. Schloss +24 more
TL;DR: A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility.
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The Potential and Challenges of Nanopore Sequencing
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