Journal ArticleDOI
Nanopore analytics: sensing of single molecules
Stefan Howorka,Zuzanna S. Siwy +1 more
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TLDR
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current, which ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules.Abstract:
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current. Reflecting its simplicity, nanopore analytics has gained popularity and can be conducted with natural protein as well as man-made polymeric and inorganic pores. The spectrum of detectable analytes ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules. Apart from being an analytical tool, nanopores have developed into a general platform technology to investigate the biophysics, physicochemistry, and chemistry of individual molecules (critical review, 310 references).read more
Citations
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Science and technology roadmap for graphene, related two-dimensional crystals, and hybrid systems
Andrea C. Ferrari,Francesco Bonaccorso,Francesco Bonaccorso,Vladimir I. Fal'ko,Konstantin S. Novoselov,Stephan Roche,Peter Bøggild,Stefano Borini,Frank H. L. Koppens,Vincenzo Palermo,Nicola M. Pugno,Nicola M. Pugno,Nicola M. Pugno,Jose A. Garrido,Roman Sordan,Alberto Bianco,Laura Ballerini,Maurizio Prato,Elefterios Lidorikis,Jani Kivioja,Claudio Marinelli,Tapani Ryhänen,Alberto F. Morpurgo,Jonathan N. Coleman,Valeria Nicolosi,Luigi Colombo,Albert Fert,Albert Fert,Mar García-Hernández,Adrian Bachtold,Grégory F. Schneider,Francisco Guinea,Cees Dekker,Matteo Barbone,Zhipei Sun,Costas Galiotis,Alexander N. Grigorenko,Gerasimos Konstantatos,Andras Kis,Mikhail I. Katsnelson,Lieven M. K. Vandersypen,A. Loiseau,Vittorio Morandi,Daniel Neumaier,Emanuele Treossi,Vittorio Pellegrini,Vittorio Pellegrini,Marco Polini,Alessandro Tredicucci,Gareth M. Williams,Byung Hee Hong,Jong Hyun Ahn,Jong Min Kim,Herbert Zirath,Bart J. van Wees,Herre S. J. van der Zant,Luigi Occhipinti,Andrea di Matteo,Ian A. Kinloch,Thomas Seyller,Etienne Quesnel,Xinliang Feng,K.B.K. Teo,Nalin Rupesinghe,Pertti Hakonen,Simon R. T. Neil,Quentin Tannock,Tomas Löfwander,Jari M. Kinaret +68 more
TL;DR: An overview of the key aspects of graphene and related materials, ranging from fundamental research challenges to a variety of applications in a large number of sectors, highlighting the steps necessary to take GRMs from a state of raw potential to a point where they might revolutionize multiple industries are provided.
Journal ArticleDOI
Rapid electronic detection of probe-specific microRNAs using thin nanopore sensors
TL;DR: A platform for the rapid electronic detection of probe-hybridized microRNAs from cellular RNA is presented, in which a target microRNA is first hybridized to a probe and enriched through binding to the viral protein p19.
Journal ArticleDOI
Synthetic Lipid Membrane Channels Formed by Designed DNA Nanostructures
Martin Langecker,Vera Arnaut,Thomas G. Martin,Jonathan List,Stephan Renner,Michael Mayer,Hendrik Dietz,Friedrich C. Simmel +7 more
TL;DR: In single-channel electrophysiological measurements, the authors found similarities to the response of natural ion channels, such as conductances on the order of 1 nanosiemens and channel gating, and show that the synthetic channels can be used to discriminate single DNA molecules.
Journal ArticleDOI
Nanopores: A journey towards DNA sequencing
TL;DR: Past and current studies related to nucleic acid biophysics are surveyed, and will hopefully provoke a discussion of immediate and future prospects for the field.
Journal ArticleDOI
Controlling protein translocation through nanopores with bio-inspired fluid walls
Erik Yusko,Jay M. Johnson,Sheereen Majd,Panchika Prangkio,Ryan Rollings,Jiali Li,Jerry Yang,Michael Mayer +7 more
TL;DR: It is shown that coating nanopores with fluid bilayer lipids allows the pore diameters to be fine-tuned in sub-nanometre increments, and incorporation of mobile ligands in the lipid conferred specificity and slowed down the translocation of targeted proteins sufficiently to time-resolve translocation events of individual proteins.
References
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Journal ArticleDOI
Direct introduction of single protein channels and pores into lipid bilayers.
Matthew A. Holden,Hagan Bayley +1 more
TL;DR: A mechanical method for inserting single pores and channels into lipid bilayers using a hand-operated hydrogel probe coated with a layer of proteins that retains electrical properties that are the same as those of proteins inserted from solution.
Journal ArticleDOI
Synthetic pores with reactive signal amplifiers as artificial tongues
Svetlana Litvinchuk,Hiroyuki Tanaka,Tomohiro Miyatake,Dario Pasini,Takatsugu Tanaka,Guillaume Bollot,Jiri Mareda,Stefan Matile +7 more
TL;DR: Synthetic pores are presented that can sense flavours in food and in addition make them visibly detectable, and reactive amplifiers that covalently capture elusive analytes after enzymatic signal generation and drag them into synthetic pores for blockage are exploited.
Journal ArticleDOI
Electromechanical Unzipping of Individual DNA Molecules Using Synthetic Sub-2 nm Pores
TL;DR: Using sub-2 nm solid-state nanopores, it is shown for the first time that the unzipping kinetics of individual DNA duplexes can be probed by analyzing the dwell-time distributions, a crucial step toward sequence variability detection and single-molecule nanopore sequencing technology, which rely on parallel detection from nanopore arrays.
Journal ArticleDOI
Fabrication of nanopores in silicon chips using feedback chemical etching
Journal ArticleDOI
Ionic conductivity of the aqueous layer separating a lipid bilayer membrane and a glass support.
TL;DR: The results indicate that the concentration and mobility of charge carriers in the aqueous layer between the glass support and bilayer are largely determined by the local structure of the glass/water/bilayer interface.
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