Journal ArticleDOI
Nanopore analytics: sensing of single molecules
Stefan Howorka,Zuzanna S. Siwy +1 more
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TLDR
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current, which ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules.Abstract:
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current. Reflecting its simplicity, nanopore analytics has gained popularity and can be conducted with natural protein as well as man-made polymeric and inorganic pores. The spectrum of detectable analytes ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules. Apart from being an analytical tool, nanopores have developed into a general platform technology to investigate the biophysics, physicochemistry, and chemistry of individual molecules (critical review, 310 references).read more
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Journal ArticleDOI
Polydimethysiloxane Modified Silica Nanochannel Membrane for Hydrophobicity-Based Molecular Filtration and Detection
TL;DR: The fabrication of ultrathin silica nanochannel membranes inhomogeneously modified by polydimethysiloxane (PDMS), designated as PDMS-SNM, for hydrophobicity-based molecular filtration and detection and an excellent analytical performance and antifouling/anti-interference ability are reported.
Journal ArticleDOI
Nanofluidics in point of care applications
Loes I. Segerink,Jan C.T. Eijkel +1 more
TL;DR: Four areas are identified where nanofluidics has brought the largest contributions: single nanopores, nanoporous membranes, nanoconfinement and the use of concentration polarization.
Journal ArticleDOI
Remote Activation of a Nanopore for High-Performance Genetic Detection Using a pH Taxis-Mimicking Mechanism
TL;DR: A simple and easy-to-operate approach to noncovalently transform aerolysin into a highly nucleic acids-sensitive nanopore, and provides insight into the nanopore engineering for biosensing, making aeroly sin applicable in genetic and epigenetic detections of long nucleic acid detections.
Journal ArticleDOI
Multiscale analysis of the effect of surface charge pattern on a nanopore's rectification and selectivity properties: From all-atom model to Poisson-Nernst-Planck.
Mónika Valiskó,Bartłomiej Matejczyk,Zoltán Ható,Tamás Kristóf,Eszter Mádai,Dávid Fertig,Dirk Gillespie,Dezső Boda +7 more
TL;DR: It is found that the values of the diffusion coefficients are sensitive to the net charge of the pore but are relatively transferable to different voltages and charge patterns with the same total charge.
Journal ArticleDOI
Delivery of Single Nanoparticles from Nanopipettes under Resistive‐Pulse Control
TL;DR: Wang et al. as mentioned in this paper developed a nanoelectrochemical methodology for controlled delivery of singlenanometer-sized particles, which is based on the resistive-pulse concept, which has been widely employed in sensing applications from singlemolecule detection to particle sizing to DNA sequencing.
References
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Journal ArticleDOI
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
TL;DR: The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches.
Journal ArticleDOI
Formation and Structure of Self-Assembled Monolayers.
TL;DR: Monolayers of alkanethiolates on gold are probably the most studied SAMs to date and offer the needed design flexibility, both at the individual molecular and at the material levels, and offer a vehicle for investigation of specific interactions at interfaces, and of the effect of increasing molecular complexity on the structure and stability of two-dimensional assemblies.
Journal ArticleDOI
Characterization of individual polynucleotide molecules using a membrane channel
TL;DR: It is shown that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane, which could in principle provide direct, high-speed detection of the sequence of bases in single molecules of DNA or RNA.
Book ChapterDOI
Ion Channels in Excitable Membranes
TL;DR: The action potential is triggered when the membrane potential, which was at the resting level, depolarizes and reaches the threshold of excitation, which triggers the action potential.
Journal ArticleDOI
The Potential and Challenges of Nanopore Sequencing
Daniel Branton,David W. Deamer,Andre Marziali,Hagan Bayley,Steven A. Benner,Thomas Z. Butler,Massimiliano Di Ventra,Slaven Garaj,Andrew Hibbs,Xiaohua Huang,Stevan B Jovanovich,Predrag S Krstic,Stuart Lindsay,Xinsheng Sean Ling,Carlos H. Mastrangelo,Amit Meller,John S. Oliver,Yuriy V. Pershin,J. Michael Ramsey,Robert Riehn,Gautam V. Soni,Vincent Tabard-Cossa,Meni Wanunu,Matthew Wiggin,Jeffery A. Schloss +24 more
TL;DR: A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility.
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