Journal ArticleDOI
Nanopore analytics: sensing of single molecules
Stefan Howorka,Zuzanna S. Siwy +1 more
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TLDR
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current, which ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules.Abstract:
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current. Reflecting its simplicity, nanopore analytics has gained popularity and can be conducted with natural protein as well as man-made polymeric and inorganic pores. The spectrum of detectable analytes ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules. Apart from being an analytical tool, nanopores have developed into a general platform technology to investigate the biophysics, physicochemistry, and chemistry of individual molecules (critical review, 310 references).read more
Citations
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Journal ArticleDOI
Nanopore-Based Protein Identification.
Mazdak Afshar Bakshloo,John J. Kasianowicz,Manuela Pastoriza-Gallego,Jérôme Mathé,Régis Daniel,Fabien Piguet,Abdelghani Oukhaled +6 more
TL;DR: It is demonstrated that the polypeptide fragments can instead be detected and classified at the single-molecule limit using a nanometer-scale pore formed by the protein aerolysin.
Journal ArticleDOI
Tunable Streaming Current in a pH-Regulated Nanochannel by a Field Effect Transistor
TL;DR: In this paper, active control of the surface charge property and the streaming current, generated by a pressure-driven flow, in a pH-regulated nanochannel using a field effect transistor (FET) is analyzed for the first time.
Journal ArticleDOI
Structure, Dynamics, and Substrate Specificity of the OprO Porin from Pseudomonas aeruginosa.
Niraj Modi,Sonalli Ganguly,Iván Bárcena-Uribarri,Roland Benz,Bert van den Berg,Ulrich Kleinekathöfer +5 more
TL;DR: Applied-field MD simulations and electrophysiological experiments demonstrated that the double mutations interchange the phosphate and diphosphate specificities of OprP and OprO, outlining a possible strategy to rationally design channel specificity by modification of a small number of residues that may be applicable to other pores as well.
Journal ArticleDOI
Multiplex quantitative detection of SARS-CoV-2 specific IgG and IgM antibodies based on DNA-assisted nanopore sensing.
Zehui Zhang,Xiaoqin Wang,Xiaojun Wei,Sophia W. Zheng,Brian Lenhart,Peisheng Xu,Jie Li,Jing Pan,Helmut Albrecht,Chang Liu +9 more
TL;DR: In this paper, the authors developed DNA-assisted nanopore sensing for quantification of SARS-CoV-2 related antibodies in human serum, using different DNA structures as detection reporters for multiplex quantification.
Journal ArticleDOI
Through a Window, Brightly: A Review of Selected Nanofabricated Thin-Film Platforms for Spectroscopy, Imaging, and Detection:
Jason R. Dwyer,Maher Harb +1 more
TL;DR: A thin-walled nanofluidic sample cell concept that has advanced the discovery horizons of ultrafast spectroscopy and of electron microscopy investigations of in-liquid samples; and a unique class of thin-film-based nanofLUidic devices, designed around a nanopore, with expansive prospects for single molecule sensing are highlighted.
References
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Journal ArticleDOI
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
TL;DR: The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches.
Journal ArticleDOI
Formation and Structure of Self-Assembled Monolayers.
TL;DR: Monolayers of alkanethiolates on gold are probably the most studied SAMs to date and offer the needed design flexibility, both at the individual molecular and at the material levels, and offer a vehicle for investigation of specific interactions at interfaces, and of the effect of increasing molecular complexity on the structure and stability of two-dimensional assemblies.
Journal ArticleDOI
Characterization of individual polynucleotide molecules using a membrane channel
TL;DR: It is shown that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane, which could in principle provide direct, high-speed detection of the sequence of bases in single molecules of DNA or RNA.
Book ChapterDOI
Ion Channels in Excitable Membranes
TL;DR: The action potential is triggered when the membrane potential, which was at the resting level, depolarizes and reaches the threshold of excitation, which triggers the action potential.
Journal ArticleDOI
The Potential and Challenges of Nanopore Sequencing
Daniel Branton,David W. Deamer,Andre Marziali,Hagan Bayley,Steven A. Benner,Thomas Z. Butler,Massimiliano Di Ventra,Slaven Garaj,Andrew Hibbs,Xiaohua Huang,Stevan B Jovanovich,Predrag S Krstic,Stuart Lindsay,Xinsheng Sean Ling,Carlos H. Mastrangelo,Amit Meller,John S. Oliver,Yuriy V. Pershin,J. Michael Ramsey,Robert Riehn,Gautam V. Soni,Vincent Tabard-Cossa,Meni Wanunu,Matthew Wiggin,Jeffery A. Schloss +24 more
TL;DR: A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility.
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