Oocyte stage-specific effects of MTOR determine granulosa cell fate and oocyte quality in mice
Jing Guo,Teng Zhang,Yueshuai Guo,Tao Sun,Hui Li,Xiaoyun Zhang,Hong Yin,Guangyi Cao,Yaoxue Yin,Hao Wang,Lanying Shi,Xuejiang Guo,Jiahao Sha,John J. Eppig,You-Qiang Su +14 more
TLDR
It is shown that conditional knockout (cKO) of Mtor in either primordial or growing oocytes caused infertility but differentially affected oocyte quality, granulosa cell fate, and follicular development.Abstract:
MTOR (mechanistic target of rapamycin) is a widely recognized integrator of signals and pathways key for cellular metabolism, proliferation, and differentiation. Here we show that conditional knockout (cKO) of Mtor in either primordial or growing oocytes caused infertility but differentially affected oocyte quality, granulosa cell fate, and follicular development. cKO of Mtor in nongrowing primordial oocytes caused defective follicular development leading to progressive degeneration of oocytes and loss of granulosa cell identity coincident with the acquisition of immature Sertoli cell-like characteristics. Although Mtor was deleted at the primordial oocyte stage, DNA damage accumulated in oocytes during their later growth, and there was a marked alteration of the transcriptome in the few oocytes that achieved the fully grown stage. Although oocyte quality and fertility were also compromised when Mtor was deleted after oocytes had begun to grow, these occurred without overtly affecting folliculogenesis or the oocyte transcriptome. Nevertheless, there was a significant change in a cohort of proteins in mature oocytes. In particular, down-regulation of PRC1 (protein regulator of cytokinesis 1) impaired completion of the first meiotic division. Therefore, MTOR-dependent pathways in primordial or growing oocytes differentially affected downstream processes including follicular development, sex-specific identity of early granulosa cells, maintenance of oocyte genome integrity, oocyte gene expression, meiosis, and preimplantation developmental competence.read more
Citations
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Newly Identified Regulators of Ovarian Folliculogenesis and Ovulation.
Eran Gershon,Nava Dekel +1 more
TL;DR: The task of this review is to define the different stages of folliculogenesis culminating at ovulation and CL formation, and to summarize the most recent information regarding the newly identified factors that regulate the specific stages of this highly intricated process.
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Role of mTOR Signaling in Female Reproduction
Zaixin Guo,Qi Yu +1 more
TL;DR: Whether strategies to improve or suppress mTOR expression could have therapeutic potential for reproductive diseases like premature ovarian failure, polycystic ovarian syndrome, and endometriosis is assessed.
Journal ArticleDOI
Characterization of Metabolic Patterns in Mouse Oocytes during Meiotic Maturation.
Ling Li,Shuai Zhu,Wenjie Shu,Yueshuai Guo,Yusheng Guan,Juan Zeng,Haichao Wang,Longsen Han,Jiaqi Zhang,Xiaohui Liu,Chunling Li,Xiaojing Hou,Min Gao,Juan Ge,Chao Ren,Hao Zhang,Tim Schedl,Xuejiang Guo,Minjian Chen,Qiang Wang +19 more
TL;DR: The temporal metabolome profiles of mouse oocytes during in-vivo maturation are obtained by isolating large number of cells at key stages by identifying the key targets mediating the action of PUFA arachidonic acid on meiotic maturation and the control of epigenetic marks in maturing oocytes by SGOC network.
Journal ArticleDOI
RNA-Binding Protein IGF2BP2/IMP2 is a Critical Maternal Activator in Early Zygotic Genome Activation.
Hongbin Liu,Tahir Muhammad,Yueshuai Guo,Mengjing Li,Qian-Qian Sha,Chuanxin Zhang,Hui Liu,Shigang Zhao,Han Zhao,Hao Zhang,Yanzhi Du,Kang Sun,Kui Liu,Gang Lu,Xuejiang Guo,Jiahao Sha,Heng-Yu Fan,Fei Gao,Zi-Jiang Chen +18 more
TL;DR: It is shown that maternal deletion of Igf 2bp2 in mouse embryos causes early embryonic developmental arrest in vitro at the 2‐cell‐stage, and the potential embryonic development‐related utility of IGF2 for animal biotechnology and for assisted reproduction in humans is revealed.
Journal ArticleDOI
The histone modification reader ZCWPW1 is required for meiosis prophase I in male but not in female mice
M. Li,M. Li,Tao Huang,Tao Huang,Mengjing Li,Mengjing Li,Chuanxin Zhang,Chuanxin Zhang,Xiaochen Yu,Xiaochen Yu,Yingying Yin,Yingying Yin,Chao Liu,Xin Wang,Haiwei Feng,Haiwei Feng,Tuo Zhang,Mo-Fang Liu,Chunsheng Han,Gang Lu,Wei Li,Jinlong Ma,Jinlong Ma,Zi-Jiang Chen,Hongbin Liu,Hongbin Liu,Kui Liu,Kui Liu +27 more
TL;DR: It is concluded that the H3K4me3 reader ZCwpW1 is indispensable for meiosis synapsis in males but is dispensable for females, and the results suggest that ZCWPW1 may represent a previously unknown, sex-dependent epigenetic regulator of germ cell meiosis in mammals.
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