Potent neutralizing antibodies from COVID-19 patients define multiple targets of vulnerability.
Philip J. M. Brouwer,Tom G. Caniels,Karlijn van der Straten,Jonne L. Snitselaar,Yoann Aldon,Sandhya Bangaru,Jonathan L. Torres,Nisreen M.A. Okba,Mathieu Claireaux,Gius Kerster,Arthur E. H. Bentlage,Marlies M. van Haaren,Denise Guerra,Judith A. Burger,Edith E. Schermer,Kirsten D. Verheul,Niels van der Velde,Alex van der Kooi,Jelle van Schooten,Mariëlle J. van Breemen,Tom P. L. Bijl,Kwinten Sliepen,Aafke Aartse,Aafke Aartse,Ronald Derking,Ilja Bontjer,Neeltje A. Kootstra,W. Joost Wiersinga,Gestur Vidarsson,Bart L. Haagmans,Andrew B. Ward,Godelieve J. de Bree,Rogier W. Sanders,Rogier W. Sanders,Marit J. van Gils +34 more
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It is shown that the patients had strong immune responses against the viral spike protein, a complex that binds to receptors on the host cell, and monoclonal antibodies isolated here are promising candidates for COVID-19 treatment and prevention.Abstract:
The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has had a large impact on global health, travel, and economy. Therefore, preventative and therapeutic measures are urgently needed. Here, we isolated monoclonal antibodies from three convalescent coronavirus disease 2019 (COVID-19) patients using a SARS-CoV-2 stabilized prefusion spike protein. These antibodies had low levels of somatic hypermutation and showed a strong enrichment in VH1-69, VH3-30-3, and VH1-24 gene usage. A subset of the antibodies was able to potently inhibit authentic SARS-CoV-2 infection at a concentration as low as 0.007 micrograms per milliliter. Competition and electron microscopy studies illustrate that the SARS-CoV-2 spike protein contains multiple distinct antigenic sites, including several receptor-binding domain (RBD) epitopes as well as non-RBD epitopes. In addition to providing guidance for vaccine design, the antibodies described here are promising candidates for COVID-19 treatment and prevention.read more
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Semantic and population analysis of the genetic targets related to COVID-19 and its association with genes and diseases
Louis Papageorgiou,Eleni Papakonstantinou,Io Diakou,Katerina Pierouli,Konstantina Dragoumani,Flora Bacopoulou,George P. Chrousos,Elias Eliopoulos,Dimitrios Vlachakis +8 more
TL;DR: A semantic analysis of SARS-CoV-2 related publications yielded a repertoire of SNPs, genes and disease ontologies, which have the potential to elucidate the complex interaction between COVID-19 pathogenesis and host genetic variation.
Peer Review
COVID-19 Infection: An Overview of Pathophysiology, Management, and Complications
TL;DR: This article is a general review, and the management guidelines are different in different parts of the World, Hence, the reader should consult local health guidelines to manage their patients.
Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies
Katrin Fischer,Aleksei Lulla,Tsz Y. So,P. Pereyra-Gerber,Matthew I. J. Raybould,Timo N Kohler,Tomasz S. Kaminski,Juan Carlos Yam-Puc,Robert Hughes,Florian Leiß-Maier,Paul Brear,Nick Matheson,Charlotte M. Deane,Marko Hyvönen,James Thaventhiran,Florian Hollfelder +15 more
TL;DR: In this paper , microfluidic encapsulation of single cells into an antibody capture hydrogel with antigen bait sorting by conventional flow cytometry was used for the rapid discovery of monoclonal antibodies from ASCs.
Journal ArticleDOI
Comparative Analysis and Classification of SARS-CoV-2 Spike Protein Structures in PDB
TL;DR: In this article , the comparative analysis of SARS-CoV-2 S protein structures is performed through comparative analysis, and the protein structures in the PDB (protein data bank) database are compared and analyzed not only with each other but with the structures of other viruses for various parameters.
Journal ArticleDOI
Neutralizing Efficacy of Encapsulin Nanoparticles against SARS-CoV2 Variants of Concern
Sara Khaleeq,Nayanika Sengupta,Sahil Kumar,Unnatiben Rajeshbhai Patel,R. S. Rajmani,Poorvi Reddy,Suman Pandey,Randhir Singh,Somnath Dutta,Rajesh P. Ringe,Raghavan Varadarajan +10 more
TL;DR: In this paper , a nanoparticle display combined with the SpyTag-SpyCatcher system was used to design encapsulin-mRBD, a vaccine displaying 180 copies of the monomeric SARS-CoV-2 spike receptor-binding domain (RBD).
References
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