Journal ArticleDOI
Predicting coiled coils from protein sequences
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TLDR
This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled -coil structures,such as the hinge region in myosin.Abstract:
The probability that a residue in a protein is part of a coiled-coil structure was assessed by comparison of its flanking sequences with sequences of known coiled-coil proteins. This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled-coil structures, such as the hinge region in myosin. More than 200 proteins that probably have coiled-coil domains were identified in GenBank, including alpha- and beta-tubulins, flagellins, G protein beta subunits, some bacterial transfer RNA synthetases, and members of the heat shock protein (Hsp70) family.read more
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An internal motor kinesin is associated with the golgi apparatus and plays a role in trichome morphogenesis in Arabidopsis
TL;DR: The identified similar internal motor kinesins, Kinesin-13A, from the cotton Gossypium hirsutum and Arabidopsis thaliana and the MCAK/Kinesin13 subfamily indicate that plant kines INS have evolved to take on different tasks than their animal counterparts.
Journal ArticleDOI
Melanophilin directly links Rab27a and myosin Va through its distinct coiled‐coil regions
Kazuaki Nagashima,Seiji Torii,Zhaohong Yi,Michihiro Igarashi,Koichi Okamoto,Toshiyuki Takeuchi,Tetsuro Izumi +6 more
TL;DR: Melanophilin has a role in bridging Rab27a on melanosomes and myosin Va on actin filaments during melanosome transport and it is proposed that the Rab‐binding region conserved in a novel rabphilin3/granuphilin‐like Rab effector family constitutes an α‐helix‐based coiled‐coil structure.
Journal ArticleDOI
Tea2p Is a Kinesin-like Protein Required to Generate Polarized Growth in Fission Yeast
TL;DR: Results suggest that this kinesin promotes microtubule growth, possibly through interactions with the microtubules end, and that it is important for establishing and maintaining polarized growth along the long axis of the cell.
Journal ArticleDOI
PDV1 and PDV2 Mediate Recruitment of the Dynamin-Related Protein ARC5 to the Plastid Division Site
TL;DR: The results indicate that plastid division involves the stepwise localization of FtsZ, PDV1, and ARC5 at the division site and that PDV 1 and PDV2 together mediate the recruitment of ARC5 to the midplastid constriction at a late stage of division.
Journal ArticleDOI
Peripheral framework of carrot cell nucleus contains a novel protein predicted to exhibit a long alpha-helical domain
K. Masuda,K. Masuda,Z.-J. Xu,Z.-J. Xu,Satoru Takahashi,Satoru Takahashi,A. Ito,A. Ito,Michiyuki Ono,Michiyuki Ono,Koji Nomura,Koji Nomura,M. Inoue,M. Inoue +13 more
TL;DR: The results suggest that NMCP1 protein forms coiled-coil filaments and is a constituent of the peripheral architecture of the higher plant cell nucleus.
References
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Journal ArticleDOI
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
TL;DR: A 30-amino-acid segment of C/EBP, a newly discovered enhancer binding protein, shares notable sequence similarity with a segment of the cellular Myc transforming protein, and may represent a characteristic property of a new category of DNA binding proteins.
Journal ArticleDOI
Evidence that the leucine zipper is a coiled coil
TL;DR: A peptide corresponding to the leucine zipper region of the yeast transcriptional activator GCN4 was synthesized and characterized and associates in the micromolar concentration range to form a very stable dimer of alpha helices with a parallel orientation.
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Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.
TL;DR: The putative DNA-binding domain of CREB is structurally similar to the corresponding domains in the phorbol ester-responsive c-jun protein and the yeast transcription factor GCN4.
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α‐Helical coiled coils and bundles: How to design an α‐helical protein
Carolyn Cohen,David A.D. Parry +1 more
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A second class of synthetase structure revealed by X-ray analysis of Escherichia coli seryl-tRNA synthetase at 2.5 A.
TL;DR: The three-dimensional crystal structure of seryl-transfer RNA synthetase from Escherichia coli, refined at 2.5 Å resolution, is described, and is the first representative of a second class of aminoacyl-tRNA synthet enzyme structures.