Journal ArticleDOI
Predicting coiled coils from protein sequences
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TLDR
This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled -coil structures,such as the hinge region in myosin.Abstract:
The probability that a residue in a protein is part of a coiled-coil structure was assessed by comparison of its flanking sequences with sequences of known coiled-coil proteins. This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled-coil structures, such as the hinge region in myosin. More than 200 proteins that probably have coiled-coil domains were identified in GenBank, including alpha- and beta-tubulins, flagellins, G protein beta subunits, some bacterial transfer RNA synthetases, and members of the heat shock protein (Hsp70) family.read more
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The inner membrane protein Mdm33 controls mitochondrial morphology in yeast
Marlies Messerschmitt,Stefan Jakobs,Frank Vogel,Stefan Fritz,Kai Stefan Dimmer,Walter Neupert,Benedikt Westermann +6 more
TL;DR: It is proposed that Mdm33 is involved in fission of the mitochondrial inner membrane, a Saccharomyces cerevisiae gene encoding a novel protein of the mitochondria that performs homotypic protein–protein interactions in the inner membrane.
Journal ArticleDOI
Structural characterization of a type III secretion system filament protein in complex with its chaperone.
TL;DR: It is shown that EspA alone is sufficient to form filamentous structures in the absence of other pathogenicity island–encoded proteins, and it is demonstrated that CesA traps EspA in a monomeric state and inhibits its polymerization.
Journal ArticleDOI
Caenorhabditis elegans HIM-18/SLX-4 interacts with SLX-1 and XPF-1 and maintains genomic integrity in the germline by processing recombination intermediates.
TL;DR: HIM-18, an ortholog of MUS312/Slx4, is identified as a critical player required in vivo for processing late HR intermediates in Caenorhabditis elegans and proposed that HIM-18 facilitates processing ofHR intermediates resulting from replication fork collapse and programmed meiotic DSBs in the C. elegans germline.
Journal ArticleDOI
Identification and localization of a neurally expressed member of the plakoglobin/armadillo multigene family.
TL;DR: In situ hybridization and immunofluorescence microscopy using NPRAP-specific antibodies, it is demonstrated NPRAP and its mRNA in the perikarya of various kinds of CNS neurons in embryonic and adult mice, but minimal amounts have also been detected by immunoblot analysis in some other tissues containing neural or neuroendocrine elements.
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The Yeast Dynactin Complex Is Involved in Partitioning the Mitotic Spindle between Mother and Daughter Cells during Anaphase B
Jason Kahana,Gabriel Schlenstedt,Darren M. Evanchuk,John R. Geiser,M. Andrew Hoyt,Pamela A. Silver +5 more
TL;DR: Analysis of spindle dynamics by time-lapse digital microscopy and genetic depletion experiments indicate that the yeast dynactin complex and cytoplasmic dynein together define a physiological pathway that is responsible for spindle translocation late in anaphase.
References
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Journal ArticleDOI
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
TL;DR: A 30-amino-acid segment of C/EBP, a newly discovered enhancer binding protein, shares notable sequence similarity with a segment of the cellular Myc transforming protein, and may represent a characteristic property of a new category of DNA binding proteins.
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Evidence that the leucine zipper is a coiled coil
TL;DR: A peptide corresponding to the leucine zipper region of the yeast transcriptional activator GCN4 was synthesized and characterized and associates in the micromolar concentration range to form a very stable dimer of alpha helices with a parallel orientation.
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Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.
TL;DR: The putative DNA-binding domain of CREB is structurally similar to the corresponding domains in the phorbol ester-responsive c-jun protein and the yeast transcription factor GCN4.
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α‐Helical coiled coils and bundles: How to design an α‐helical protein
Carolyn Cohen,David A.D. Parry +1 more
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A second class of synthetase structure revealed by X-ray analysis of Escherichia coli seryl-tRNA synthetase at 2.5 A.
TL;DR: The three-dimensional crystal structure of seryl-transfer RNA synthetase from Escherichia coli, refined at 2.5 Å resolution, is described, and is the first representative of a second class of aminoacyl-tRNA synthet enzyme structures.