Journal ArticleDOI
Predicting coiled coils from protein sequences
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TLDR
This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled -coil structures,such as the hinge region in myosin.Abstract:
The probability that a residue in a protein is part of a coiled-coil structure was assessed by comparison of its flanking sequences with sequences of known coiled-coil proteins. This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled-coil structures, such as the hinge region in myosin. More than 200 proteins that probably have coiled-coil domains were identified in GenBank, including alpha- and beta-tubulins, flagellins, G protein beta subunits, some bacterial transfer RNA synthetases, and members of the heat shock protein (Hsp70) family.read more
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Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast, Saccharomyces cerevisiae.
TL;DR: It is found that overproduction of Apg14p partially reversed the defect in autophagy induced by the apg6-1 mutation, implying that Apg6/Vps30p has two distinct functions in the autophagic process and the vacuolar protein sorting pathway.
Journal ArticleDOI
The t(8;9)(p22;p24) is a recurrent abnormality in chronic and acute leukemia that fuses PCM1 to JAK2
Andreas Reiter,Christoph Walz,Ann E. Watmore,Claudia Schoch,Ilona Blau,Brigitte Schlegelberger,Ute Berger,Nick Telford,Shilani Aruliah,John A Yin,Danny Vanstraelen,Helen F Barker,Peter C Taylor,Aisling O'Driscoll,Fabio Benedetti,Cornelia Rudolph,Hans-Jochem Kolb,Andreas Hochhaus,Rüdiger Hehlmann,Andrew Chase,Nicholas C.P. Cross +20 more
TL;DR: It is concluded that human autoantigen pericentriolar material (PCM1)-JAK2 is a novel, recurrent fusion gene in hematologic malignancies and patients with PCM1- JAK2 disease are attractive candidates for targeted signal transduction therapy.
Journal ArticleDOI
βiv Spectrin, a New Spectrin Localized at Axon Initial Segments and Nodes of Ranvier in the Central and Peripheral Nervous System
Stanny Berghs,Diego Aggujaro,Ronald Dirkx,Elena Maksimova,Paul R. Stabach,Jean-Michel Hermel,Jian Ping Zhang,William M. Philbrick,Vladimir I. Slepnev,Tatiana Ort,Michele Solimena +10 more
TL;DR: It is suggested that βIVΣ1 spectrin interacts with ankyrinG 480/270-kD and participates in the clustering of voltage-gated Na+ channels and cell-adhesion molecules at initial segments and nodes of Ranvier.
Journal ArticleDOI
The S. pombe cdc15 gene is a key element in the reorganization of F-actin at mitosis
Christian Fankhauser,Alexandre Reymond,Lorenzo Cerutti,Suzan Utzig,Kay Hofmann,Viesturs Simanis +5 more
TL;DR: The earliest step in septum formation and cytokinesis, rearrangement of actin to the center of the cell, is associated with appearance of hypophosphorylated cdc15p and formation of a cDC15p ring, which colocalizes with actin.
Journal ArticleDOI
Pericentriolar material structure and dynamics.
TL;DR: A centrosome consists of two barrel-shaped centrioles embedded in a matrix of proteins known as the pericentriolar material, which serves as a platform for protein complexes that regulate organelle trafficking, protein degradation and spindle assembly.
References
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Journal ArticleDOI
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
TL;DR: A 30-amino-acid segment of C/EBP, a newly discovered enhancer binding protein, shares notable sequence similarity with a segment of the cellular Myc transforming protein, and may represent a characteristic property of a new category of DNA binding proteins.
Journal ArticleDOI
Evidence that the leucine zipper is a coiled coil
TL;DR: A peptide corresponding to the leucine zipper region of the yeast transcriptional activator GCN4 was synthesized and characterized and associates in the micromolar concentration range to form a very stable dimer of alpha helices with a parallel orientation.
Journal ArticleDOI
Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.
TL;DR: The putative DNA-binding domain of CREB is structurally similar to the corresponding domains in the phorbol ester-responsive c-jun protein and the yeast transcription factor GCN4.
Journal ArticleDOI
α‐Helical coiled coils and bundles: How to design an α‐helical protein
Carolyn Cohen,David A.D. Parry +1 more
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A second class of synthetase structure revealed by X-ray analysis of Escherichia coli seryl-tRNA synthetase at 2.5 A.
TL;DR: The three-dimensional crystal structure of seryl-transfer RNA synthetase from Escherichia coli, refined at 2.5 Å resolution, is described, and is the first representative of a second class of aminoacyl-tRNA synthet enzyme structures.