Journal ArticleDOI
Predicting coiled coils from protein sequences
Reads0
Chats0
TLDR
This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled -coil structures,such as the hinge region in myosin.Abstract:
The probability that a residue in a protein is part of a coiled-coil structure was assessed by comparison of its flanking sequences with sequences of known coiled-coil proteins. This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled-coil structures, such as the hinge region in myosin. More than 200 proteins that probably have coiled-coil domains were identified in GenBank, including alpha- and beta-tubulins, flagellins, G protein beta subunits, some bacterial transfer RNA synthetases, and members of the heat shock protein (Hsp70) family.read more
Citations
More filters
Journal ArticleDOI
Conservation of structural motifs and antigenic diversity in the Plasmodium falciparum merozoite surface protein-3 (MSP-3)
Damian J. McColl,Robin F. Anders +1 more
TL;DR: In this paper, an analysis of the merozoite surface protein-3 (MSP-3) gene from four P. falciparum isolates showed that polymorphism in msp-3 is predominantly due to sequence diversity in the N-terminal half of the predicted polypeptide within and flanking the heptad-repeat.
Journal ArticleDOI
BRANCHED1: A Key Hub of Shoot Branching.
Ming Wang,Marie-Anne Le Moigne,Jessica Bertheloot,Laurent Crespel,Maria Dolores Perez-Garcia,Laurent Ogé,Sabine Demotes-Mainard,Latifa Hamama,Jean-Michel Davière,Soulaiman Sakr +9 more
TL;DR: This review highlights the central role of BRC1 in shoot branching and its responsiveness to different stimuli, and emphasizes the different knowledge gaps that should be addressed in the near future.
Journal ArticleDOI
p150Glued, the largest subunit of the dynactin complex, is nonessential in Neurospora but required for nuclear distribution.
TL;DR: A genetic screen for cytoplasmic dynein/dynactin mutants in the filamentous fungus Neurospora crassa is devised and it is reported that one of these mutants, ro-3, defines a gene encoding an apparent homologue of p150Glued, indicating that dynactin is not an essential complex in N. crASSa.
Journal ArticleDOI
Signals, Motors, Morphogenesis - the Cytoskeleton in Plant Development*
TL;DR: The signal-triggered dynamic response of the cytoskeleton must be based on spatial cues that organize assembly and disassembly of tu-bulin and actin, and the great morphogenetic potential of cytoskeletal motors is discussed.
Journal ArticleDOI
Activating CARD14 Mutations Are Associated with Generalized Pustular Psoriasis but Rarely Account for Familial Recurrence in Psoriasis Vulgaris
Dorottya M. Berki,Lu Liu,Siew Eng Choon,A. David Burden,Christopher E.M. Griffiths,Alexander A. Navarini,Eugene S. Tan,Alan D. Irvine,Alan D. Irvine,Annamari Ranki,Takeshi Ogo,Gabriela Petrof,Satveer K. Mahil,Michael Duckworth,Michael H. Allen,Pasquale Vito,Richard C. Trembath,John A. McGrath,Catherine H. Smith,Francesca Capon,Jonathan Barker +20 more
TL;DR: A systematic CARD14 analysis in an extended patient cohort indicated that the analysis of CARD14 mutations could help stratify pustular psoriasis cohorts but would be mostly uninformative in the context of Psoriasis and sporadic PRP.
References
More filters
Journal ArticleDOI
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
TL;DR: A 30-amino-acid segment of C/EBP, a newly discovered enhancer binding protein, shares notable sequence similarity with a segment of the cellular Myc transforming protein, and may represent a characteristic property of a new category of DNA binding proteins.
Journal ArticleDOI
Evidence that the leucine zipper is a coiled coil
TL;DR: A peptide corresponding to the leucine zipper region of the yeast transcriptional activator GCN4 was synthesized and characterized and associates in the micromolar concentration range to form a very stable dimer of alpha helices with a parallel orientation.
Journal ArticleDOI
Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.
TL;DR: The putative DNA-binding domain of CREB is structurally similar to the corresponding domains in the phorbol ester-responsive c-jun protein and the yeast transcription factor GCN4.
Journal ArticleDOI
α‐Helical coiled coils and bundles: How to design an α‐helical protein
Carolyn Cohen,David A.D. Parry +1 more
Journal ArticleDOI
A second class of synthetase structure revealed by X-ray analysis of Escherichia coli seryl-tRNA synthetase at 2.5 A.
TL;DR: The three-dimensional crystal structure of seryl-transfer RNA synthetase from Escherichia coli, refined at 2.5 Å resolution, is described, and is the first representative of a second class of aminoacyl-tRNA synthet enzyme structures.