Journal ArticleDOI
Predicting coiled coils from protein sequences
Reads0
Chats0
TLDR
This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled -coil structures,such as the hinge region in myosin.Abstract:
The probability that a residue in a protein is part of a coiled-coil structure was assessed by comparison of its flanking sequences with sequences of known coiled-coil proteins. This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled-coil structures, such as the hinge region in myosin. More than 200 proteins that probably have coiled-coil domains were identified in GenBank, including alpha- and beta-tubulins, flagellins, G protein beta subunits, some bacterial transfer RNA synthetases, and members of the heat shock protein (Hsp70) family.read more
Citations
More filters
Journal ArticleDOI
Role of the nucleophosmin (NPM) portion of the non-Hodgkin's lymphoma-associated NPM-anaplastic lymphoma kinase fusion protein in oncogenesis
TL;DR: The activation of the truncated ALK protein by a completely heterologous oligomerization domain suggests that the functionally important role of the NPM segment of NPM-ALK in transformation is restricted to the formation of kinase-active oligomers and does not involve the alteration of normal NPM functions.
Journal ArticleDOI
Modulation of the Escherichia coli sigmaE (RpoE) heat-shock transcription-factor activity by the RseA, RseB and RseC proteins.
TL;DR: The σE (RpoE) transcription factor of Escherichia coli regulates the expression of genes whose products are devoted to extracytoplasmic activities and is further modulated by the products of the remaining genes in this operon, rseB and rseC.
Journal ArticleDOI
A novel RING finger protein interacts with the cytoplasmic domain of CD40.
TL;DR: This work utilizes the yeast two-hybrid system to clone cDNAs encoding proteins that bind the CD40 cytoplasmic domain and identifies the first identification of a molecule that interacts with CD40 and whose sequence suggests a potential role in signaling.
Journal ArticleDOI
Comparative genomics enabled the isolation of the R3a late blight resistance gene in potato.
Sanwen Huang,Edwin A. G. van der Vossen,Hanhui Kuang,Vivianne G. A. A. Vleeshouwers,Ningwen Zhang,Theo Borm,Herman J. van Eck,Barbara Baker,Evert Jacobsen,Richard G. F. Visser +9 more
TL;DR: A local RGA approach was adopted using genomic information from the model Solanaceous plant tomato to isolate R3a, a potato gene that confers race-specific resistance to the late blight pathogen Phytophthora infestans and some of its paralogues whose functions remain unknown.
Journal ArticleDOI
Dual Role of Mitofilin in Mitochondrial Membrane Organization and Protein Biogenesis
Karina von der Malsburg,Judith M. Müller,Maria Bohnert,Silke Oeljeklaus,Paulina Kwiatkowska,Paulina Kwiatkowska,Thomas Becker,Adrianna Loniewska-Lwowska,Sebastian Wiese,Sanjana Rao,Dusanka Milenkovic,Dana P. Hutu,Ralf M. Zerbes,Agnes Schulze-Specking,Helmut E. Meyer,Jean-Claude Martinou,Sabine Rospert,Peter Rehling,Peter Rehling,Chris Meisinger,Marten Veenhuis,Bettina Warscheid,Ida J. van der Klei,Nikolaus Pfanner,Agnieszka Chacinska,Agnieszka Chacinska,Martin van der Laan +26 more
TL;DR: The findings indicate that mitofilin is a central component of MINOS and functions as a multifunctional regulator of mitochondrial architecture and protein biogenesis.
References
More filters
Journal ArticleDOI
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
TL;DR: A 30-amino-acid segment of C/EBP, a newly discovered enhancer binding protein, shares notable sequence similarity with a segment of the cellular Myc transforming protein, and may represent a characteristic property of a new category of DNA binding proteins.
Journal ArticleDOI
Evidence that the leucine zipper is a coiled coil
TL;DR: A peptide corresponding to the leucine zipper region of the yeast transcriptional activator GCN4 was synthesized and characterized and associates in the micromolar concentration range to form a very stable dimer of alpha helices with a parallel orientation.
Journal ArticleDOI
Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.
TL;DR: The putative DNA-binding domain of CREB is structurally similar to the corresponding domains in the phorbol ester-responsive c-jun protein and the yeast transcription factor GCN4.
Journal ArticleDOI
α‐Helical coiled coils and bundles: How to design an α‐helical protein
Carolyn Cohen,David A.D. Parry +1 more
Journal ArticleDOI
A second class of synthetase structure revealed by X-ray analysis of Escherichia coli seryl-tRNA synthetase at 2.5 A.
TL;DR: The three-dimensional crystal structure of seryl-transfer RNA synthetase from Escherichia coli, refined at 2.5 Å resolution, is described, and is the first representative of a second class of aminoacyl-tRNA synthet enzyme structures.