Journal ArticleDOI
Predicting coiled coils from protein sequences
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TLDR
This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled -coil structures,such as the hinge region in myosin.Abstract:
The probability that a residue in a protein is part of a coiled-coil structure was assessed by comparison of its flanking sequences with sequences of known coiled-coil proteins. This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled-coil structures, such as the hinge region in myosin. More than 200 proteins that probably have coiled-coil domains were identified in GenBank, including alpha- and beta-tubulins, flagellins, G protein beta subunits, some bacterial transfer RNA synthetases, and members of the heat shock protein (Hsp70) family.read more
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Interaction Between Hamartin and Tuberin, the TSC1 and TSC2 Gene Products
Marjon van Slegtenhorst,Mark Nellist,Bas Nagelkerken,Jeremy Peter Cheadle,Russell G. Snell,Ans M.W. van den Ouweland,Arnold J. J. Reuser,Julian R. Sampson,Dicky J. J. Halley,Peter van der Sluijs +9 more
TL;DR: It is shown that hamartin and tuberin associate physically in vivo and that the interaction is mediated by predicted coiled-coil domains, suggesting that hamartsin and tubulin function in the same complex rather than in separate pathways.
Journal ArticleDOI
Mutations in myosin heavy chain 11 cause a syndrome associating thoracic aortic aneurysm/aortic dissection and patent ductus arteriosus
Limin Zhu,Roger Vranckx,Philippe Khau Van Kien,Philippe Khau Van Kien,Alain Lalande,Nicolas Boisset,Nicolas Boisset,Flavie Mathieu,Flavie Mathieu,Mark Wegman,Luke Glancy,Jean-Marie Gasc,Jean-Marie Gasc,François Brunotte,Patrick Bruneval,Jean-Eric Wolf,Jean-Baptiste Michel,Xavier Jeunemaitre +17 more
TL;DR: Human MYH11 gene mutations provide the first example of a direct change in a specific SMC protein leading to an inherited arterial disease.
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Human T cell leukemia virus type 1 oncoprotein Tax targets the human mitotic checkpoint protein MAD1
TL;DR: A model of viral transformation in which Tax targets TXBP181, thereby abrogating a mitotic checkpoint is proposed, which proposes a phenotype consistent with a loss of HsMAD1 function.
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Smart polymeric gels : Redefining the limits of biomedical devices
TL;DR: The barriers in the current performance potential of biomedical devices can be lowered or removed by the rapid convergence of interdisciplinary technologies, involving supramolecular chemistry.
Journal ArticleDOI
PALB2 is an integral component of the BRCA complex required for homologous recombination repair
TL;DR: PALB2 is uncovered as the molecular adaptor between the BRCA proteins, and it is suggested that impaired HR repair is one of the fundamental causes for genomic instability and tumorigenesis observed in patients carrying BRC a1, BRCa2, or PALB2 mutations.
References
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Journal ArticleDOI
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
TL;DR: A 30-amino-acid segment of C/EBP, a newly discovered enhancer binding protein, shares notable sequence similarity with a segment of the cellular Myc transforming protein, and may represent a characteristic property of a new category of DNA binding proteins.
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Evidence that the leucine zipper is a coiled coil
TL;DR: A peptide corresponding to the leucine zipper region of the yeast transcriptional activator GCN4 was synthesized and characterized and associates in the micromolar concentration range to form a very stable dimer of alpha helices with a parallel orientation.
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Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.
TL;DR: The putative DNA-binding domain of CREB is structurally similar to the corresponding domains in the phorbol ester-responsive c-jun protein and the yeast transcription factor GCN4.
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α‐Helical coiled coils and bundles: How to design an α‐helical protein
Carolyn Cohen,David A.D. Parry +1 more
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A second class of synthetase structure revealed by X-ray analysis of Escherichia coli seryl-tRNA synthetase at 2.5 A.
TL;DR: The three-dimensional crystal structure of seryl-transfer RNA synthetase from Escherichia coli, refined at 2.5 Å resolution, is described, and is the first representative of a second class of aminoacyl-tRNA synthet enzyme structures.