Journal ArticleDOI
Predicting coiled coils from protein sequences
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TLDR
This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled -coil structures,such as the hinge region in myosin.Abstract:
The probability that a residue in a protein is part of a coiled-coil structure was assessed by comparison of its flanking sequences with sequences of known coiled-coil proteins. This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled-coil structures, such as the hinge region in myosin. More than 200 proteins that probably have coiled-coil domains were identified in GenBank, including alpha- and beta-tubulins, flagellins, G protein beta subunits, some bacterial transfer RNA synthetases, and members of the heat shock protein (Hsp70) family.read more
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Deletions in the repertoire of Pseudomonas syringae pv. tomato DC3000 type III secretion effector genes reveal functional overlap among effectors.
Brian H. Kvitko,Duck Hwan Park,André C. Velásquez,Chia-Fong Wei,Alistair B. Russell,Gregory B. Martin,Gregory B. Martin,David J. Schneider,David J. Schneider,Alan Collmer +9 more
TL;DR: The existence of an AvrE/DspA/E/HopR effector superfamily, which has representatives in virtually all groups of proteobacterial plant pathogens that deploy type III effectors, was revealed through bioinformatic analysis.
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Protein composition of cornified cell envelopes of epidermal keratinocytes
TL;DR: The amino acid compositions of isolated CEs are determined and then modelled in terms of linear combinations of the candidate proteins, showing that stratum corneum CEs have a loricrin content of 65-70% (w/w) in human, and 80-85% in mouse.
Journal ArticleDOI
Fifty years of coiled-coils and alpha-helical bundles: a close relationship between sequence and structure.
TL;DR: Functionally, the concept that a coiled coil can act only as a static rod is no longer valid, and the range of roles that these structures have now been shown to exhibit has expanded rapidly in recent years.
Journal ArticleDOI
Role of Drosophila IKK gamma in a toll-independent antibacterial immune response.
Sophie Rutschmann,Alain C. Jung,Rui Zhou,Neal S. Silverman,Jules A. Hoffmann,Dominique Ferrandon +5 more
TL;DR: In this paper, a loss-of-function mutants were generated in the Drosophila homolog of the mammalian I-κB kinase (IKK) complex component IKKγ (also called NEMO), which is required for the Relish-dependent immune induction of the genes encoding antibacterial peptides and resistance to infections by Escherichia coli.
Journal ArticleDOI
Specific sequences in the fragile X syndrome protein FMR1 and the FXR proteins mediate their binding to 60S ribosomal subunits and the interactions among them.
TL;DR: The association of the FMR1, FXR1, and FXR2 proteins with ribosomes suggests they have functions in translation or mRNA stability, and delineated the regions of F MR1 that mediate its binding to 60S ribosomal subunits and the interactions among theFMR1-FXR family members.
References
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Journal ArticleDOI
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
TL;DR: A 30-amino-acid segment of C/EBP, a newly discovered enhancer binding protein, shares notable sequence similarity with a segment of the cellular Myc transforming protein, and may represent a characteristic property of a new category of DNA binding proteins.
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Evidence that the leucine zipper is a coiled coil
TL;DR: A peptide corresponding to the leucine zipper region of the yeast transcriptional activator GCN4 was synthesized and characterized and associates in the micromolar concentration range to form a very stable dimer of alpha helices with a parallel orientation.
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Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.
TL;DR: The putative DNA-binding domain of CREB is structurally similar to the corresponding domains in the phorbol ester-responsive c-jun protein and the yeast transcription factor GCN4.
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α‐Helical coiled coils and bundles: How to design an α‐helical protein
Carolyn Cohen,David A.D. Parry +1 more
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A second class of synthetase structure revealed by X-ray analysis of Escherichia coli seryl-tRNA synthetase at 2.5 A.
TL;DR: The three-dimensional crystal structure of seryl-transfer RNA synthetase from Escherichia coli, refined at 2.5 Å resolution, is described, and is the first representative of a second class of aminoacyl-tRNA synthet enzyme structures.