Journal ArticleDOI
Predicting coiled coils from protein sequences
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TLDR
This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled -coil structures,such as the hinge region in myosin.Abstract:
The probability that a residue in a protein is part of a coiled-coil structure was assessed by comparison of its flanking sequences with sequences of known coiled-coil proteins. This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled-coil structures, such as the hinge region in myosin. More than 200 proteins that probably have coiled-coil domains were identified in GenBank, including alpha- and beta-tubulins, flagellins, G protein beta subunits, some bacterial transfer RNA synthetases, and members of the heat shock protein (Hsp70) family.read more
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The Sec34/35 Golgi transport complex is related to the exocyst, defining a family of complexes involved in multiple steps of membrane traffic.
James R.C. Whyte,Sean Munro +1 more
TL;DR: Several of the proteins involved in vesicle targeting to the yeast Golgi apparatus show distant homology to components of two other putative tethering complexes, the exocyst and the Vps52/53/54 complex, revealing that tethering factors involved in different membrane traffic steps are structurally related.
Journal ArticleDOI
mre11S—a yeast mutation that blocks double-strand-break processing and permits nonhomologous synapsis in meiosis
Knud Nairz,Franz Klein +1 more
TL;DR: It is proposed that the unprocessed DSBs present in mre11S allow for synapsis, but that homologoussynapsis is only ensured at a later stage of recombination.
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The Ras GTPase-activating-protein-related human protein IQGAP2 harbors a potential actin binding domain and interacts with calmodulin and Rho family GTPases.
S Brill,Shihong Li,C W Lyman,D M Church,John J. Wasmuth,Lawrence Weissbach,Andre Bernards,Snijders Aj +7 more
TL;DR: Although IQGAP2 shows no GAP activity towards Cdc42 and Rac1, the protein did inhibit both the intrinsic and RhoGAP-stimulated GTP hydrolysis rates of Cdc 42 and Rac 1, suggesting an alternative mechanism via which IQG APs might modulate signaling by these GTPases.
Journal ArticleDOI
CDK5RAP2 Is a Pericentriolar Protein That Functions in Centrosomal Attachment of the γ-Tubulin Ring Complex
TL;DR: Together, CDK5RAP2 is a pericentriolar structural component that functions in gammaTuRC attachment and therefore in the microtubule organizing function of the centrosome, suggesting that centrosomes malfunction due to the CDK 5R AP2 mutations may underlie autosomal recessive primary microcephaly.
Journal ArticleDOI
MYH11 mutations result in a distinct vascular pathology driven by insulin-like growth factor 1 and angiotensin II
Hariyadarshi Pannu,Van Tran-Fadulu,Christina L. Papke,Steve Scherer,Yaozhong Liu,Caroline Presley,Dongchuan Guo,Anthony L. Estrera,Hazim J. Safi,Allan R. Brasier,G. Wesley Vick,Ali J. Marian,Chander Raman,L. Maximilian Buja,Dianna M. Milewicz +14 more
TL;DR: Data suggest that MYH11 mutations are likely to be specific to the phenotype of TAAD/PDA and result in a distinct aortic and occlusive vascular pathology potentially driven by IGF-1 and Ang II.
References
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Journal ArticleDOI
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
TL;DR: A 30-amino-acid segment of C/EBP, a newly discovered enhancer binding protein, shares notable sequence similarity with a segment of the cellular Myc transforming protein, and may represent a characteristic property of a new category of DNA binding proteins.
Journal ArticleDOI
Evidence that the leucine zipper is a coiled coil
TL;DR: A peptide corresponding to the leucine zipper region of the yeast transcriptional activator GCN4 was synthesized and characterized and associates in the micromolar concentration range to form a very stable dimer of alpha helices with a parallel orientation.
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Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.
TL;DR: The putative DNA-binding domain of CREB is structurally similar to the corresponding domains in the phorbol ester-responsive c-jun protein and the yeast transcription factor GCN4.
Journal ArticleDOI
α‐Helical coiled coils and bundles: How to design an α‐helical protein
Carolyn Cohen,David A.D. Parry +1 more
Journal ArticleDOI
A second class of synthetase structure revealed by X-ray analysis of Escherichia coli seryl-tRNA synthetase at 2.5 A.
TL;DR: The three-dimensional crystal structure of seryl-transfer RNA synthetase from Escherichia coli, refined at 2.5 Å resolution, is described, and is the first representative of a second class of aminoacyl-tRNA synthet enzyme structures.