Journal ArticleDOI
Predicting coiled coils from protein sequences
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TLDR
This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled -coil structures,such as the hinge region in myosin.Abstract:
The probability that a residue in a protein is part of a coiled-coil structure was assessed by comparison of its flanking sequences with sequences of known coiled-coil proteins. This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled-coil structures, such as the hinge region in myosin. More than 200 proteins that probably have coiled-coil domains were identified in GenBank, including alpha- and beta-tubulins, flagellins, G protein beta subunits, some bacterial transfer RNA synthetases, and members of the heat shock protein (Hsp70) family.read more
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The Golgi-localization of yeast Emp47p depends on its di-lysine motif but is not affected by the ret1-1 mutation in alpha-COP.
TL;DR: The Saccharomyces cerevisiae EMP47 gene encodes a nonessential type-I transmembrane protein with sequence homology to a class of intracellular lectins defined by ERGIC-53 and VIP36 and requires a di-lysine motif for its alpha-COP-independent, steady state localization in the Golgi.
Journal ArticleDOI
Role of acetylated human AP-endonuclease (APE1/Ref-1) in regulation of the parathyroid hormone gene
TL;DR: Results indicate that acetylation of APE1 plays an important role in this key repair protein's action in transcriptional regulation.
Journal ArticleDOI
Human myosin VIIA responsible for the Usher 1B syndrome: a predicted membrane-associated motor protein expressed in developing sensory epithelia.
Dominique Weil,Gallia Levy,Iman Sahly,Fabienne Levi-Acobas,Stéphane Blanchard,Aziz El-Amraoui,Fabien Crozet,Hervé Philippe,Marc Abitbol,Christine Petit +9 more
TL;DR: The reconstituted cDNA sequence presented here predicts a 2215 amino acid protein with a typical unconventional myosin structure that is expected to dimerize into a two-headed molecule in patients with Usher syndrome type III.
Journal ArticleDOI
OPA1 (Kjer Type) Dominant Optic Atrophy: A Novel Mitochondrial Disease
TL;DR: The findings that OPA1-type DOA, as Leber optic neuropathy, is caused by the impairment of a mitochondrial protein address the question of the vulnerability of the retinal ganglion cell in response to mitochondrial defects.
Journal ArticleDOI
Whole-genome sequencing of Oryza brachyantha reveals mechanisms underlying Oryza genome evolution
Jinfeng Chen,Quanfei Huang,Dongying Gao,Junyi Wang,Yongshan Lang,Tieyan Liu,Bo Li,Zetao Bai,Jose Luis Goicoechea,Chengzhi Liang,Chengbin Chen,Wenli Zhang,Shouhong Sun,Yi Liao,Xuemei Zhang,Lu Yang,Chengli Song,Meijiao Wang,Jinfeng Shi,Geng Liu,Junjie Liu,Heling Zhou,Weili Zhou,Qiulin Yu,Na An,Yan Chen,Qingle Cai,Bo Wang,Binghang Liu,Jiumeng Min,Ying Huang,Honglong Wu,Zhenyu Li,Yong Zhang,Ye Yin,Wenqin Song,Jiming Jiang,Scott A. Jackson,Rod A. Wing,Jun Wang,Jun Wang,Mingsheng Chen +41 more
TL;DR: In this paper, the authors reported the 261-Mb de novo assembled genome sequence of Oryza brachyantha and analyzed the breakpoints of non-collinear genes.
References
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Journal ArticleDOI
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
TL;DR: A 30-amino-acid segment of C/EBP, a newly discovered enhancer binding protein, shares notable sequence similarity with a segment of the cellular Myc transforming protein, and may represent a characteristic property of a new category of DNA binding proteins.
Journal ArticleDOI
Evidence that the leucine zipper is a coiled coil
TL;DR: A peptide corresponding to the leucine zipper region of the yeast transcriptional activator GCN4 was synthesized and characterized and associates in the micromolar concentration range to form a very stable dimer of alpha helices with a parallel orientation.
Journal ArticleDOI
Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.
TL;DR: The putative DNA-binding domain of CREB is structurally similar to the corresponding domains in the phorbol ester-responsive c-jun protein and the yeast transcription factor GCN4.
Journal ArticleDOI
α‐Helical coiled coils and bundles: How to design an α‐helical protein
Carolyn Cohen,David A.D. Parry +1 more
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A second class of synthetase structure revealed by X-ray analysis of Escherichia coli seryl-tRNA synthetase at 2.5 A.
TL;DR: The three-dimensional crystal structure of seryl-transfer RNA synthetase from Escherichia coli, refined at 2.5 Å resolution, is described, and is the first representative of a second class of aminoacyl-tRNA synthet enzyme structures.