Journal ArticleDOI
Predicting coiled coils from protein sequences
Reads0
Chats0
TLDR
This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled -coil structures,such as the hinge region in myosin.Abstract:
The probability that a residue in a protein is part of a coiled-coil structure was assessed by comparison of its flanking sequences with sequences of known coiled-coil proteins. This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled-coil structures, such as the hinge region in myosin. More than 200 proteins that probably have coiled-coil domains were identified in GenBank, including alpha- and beta-tubulins, flagellins, G protein beta subunits, some bacterial transfer RNA synthetases, and members of the heat shock protein (Hsp70) family.read more
Citations
More filters
Journal ArticleDOI
Protease resistance of syntaxin-snap-25-vamp complexes : implications for assembly and structure
Michelle A. Poirier,Joe C. Hao,Per Malkus,Charles Chan,Michael F. Moore,David S. King,Mark K. Bennett +6 more
TL;DR: Results suggest that the transmembrane domains of VAMP2 and syntaxin 1A contribute to complex assembly and stability and that amino- and carboxyl-terminal regions of SNAP-25 may function as independent domains.
Journal ArticleDOI
The Membrane Transport Factor TAP/p115 Cycles between the Golgi and Earlier Secretory Compartments and Contains Distinct Domains Required for Its Localization and Function
David S. Nelson,Cecilia Alvarez,Ya-sheng Gao,Rafael Garcia-Mata,Elizabeth Fialkowski,Elizabeth Sztul +5 more
TL;DR: It is shown that in interphase cells, TAP/p115 localizes predominantly to the Golgi and to peripheral structures that represent vesicular tubular clusters (VTCs) involved in ER to Golgi transport intermediates.
Journal ArticleDOI
Coiled Coils Direct Assembly of a Cold-Activated TRP Channel
TL;DR: It is shown that the TRPM8 cytoplasmic C-terminal domain contains a coiled coil that is necessary for channel assembly and sufficient for tetramer formation and a new tool that should be of general use in dissecting TRPM channel function is delineated.
Journal ArticleDOI
SPR28, a sixth member of the septin gene family in Saccharomyces cerevisiae that is expressed specifically in sporulating cells.
TL;DR: The characterization of SPR28 is reported, a sixth member of the S. cerevisiae septin gene family whose existence was revealed by the yeast genome project and exhibited specific two-hybrid interactions with itself and with three other septins that are expressed in sporulating cells.
Journal ArticleDOI
Sec35p, a Novel Peripheral Membrane Protein, Is Required for ER to Golgi Vesicle Docking
TL;DR: Using a cell-free assay that measures distinct steps in vesicle transport from the ER to the Golgi, it is found Sec35p is required for a vesicles docking stage catalyzed by Uso1p, suggesting that Sec 35p acts upstream of, or in conjunction with, Ypt1p and Sly1p as was previously found for Uso 1p.
References
More filters
Journal ArticleDOI
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
TL;DR: A 30-amino-acid segment of C/EBP, a newly discovered enhancer binding protein, shares notable sequence similarity with a segment of the cellular Myc transforming protein, and may represent a characteristic property of a new category of DNA binding proteins.
Journal ArticleDOI
Evidence that the leucine zipper is a coiled coil
TL;DR: A peptide corresponding to the leucine zipper region of the yeast transcriptional activator GCN4 was synthesized and characterized and associates in the micromolar concentration range to form a very stable dimer of alpha helices with a parallel orientation.
Journal ArticleDOI
Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.
TL;DR: The putative DNA-binding domain of CREB is structurally similar to the corresponding domains in the phorbol ester-responsive c-jun protein and the yeast transcription factor GCN4.
Journal ArticleDOI
α‐Helical coiled coils and bundles: How to design an α‐helical protein
Carolyn Cohen,David A.D. Parry +1 more
Journal ArticleDOI
A second class of synthetase structure revealed by X-ray analysis of Escherichia coli seryl-tRNA synthetase at 2.5 A.
TL;DR: The three-dimensional crystal structure of seryl-transfer RNA synthetase from Escherichia coli, refined at 2.5 Å resolution, is described, and is the first representative of a second class of aminoacyl-tRNA synthet enzyme structures.