Journal ArticleDOI
Predicting coiled coils from protein sequences
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TLDR
This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled -coil structures,such as the hinge region in myosin.Abstract:
The probability that a residue in a protein is part of a coiled-coil structure was assessed by comparison of its flanking sequences with sequences of known coiled-coil proteins. This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled-coil structures, such as the hinge region in myosin. More than 200 proteins that probably have coiled-coil domains were identified in GenBank, including alpha- and beta-tubulins, flagellins, G protein beta subunits, some bacterial transfer RNA synthetases, and members of the heat shock protein (Hsp70) family.read more
Citations
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A heptad motif of leucine residues found in membrane proteins can drive self-assembly of artificial transmembrane segments.
TL;DR: The results support various lines of previously reported evidence where these transmembrane segments were implicated in the oligomeric assembly of the corresponding proteins.
Journal ArticleDOI
Comparison of antiparallel and parallel two-stranded alpha-helical coiled-coils. Design, synthesis, and characterization.
TL;DR: Urea and guanidine hydrochloride denaturation studies, in conjunction with molecular modeling studies, suggest that there are no physical restrictions to the packing of hydrophobic residues in an antiparallel coiled-coil, however, interchain electrostatic interactions can have positive or negative contributions to the overall stability of the disulfide-bridged coil.
Journal ArticleDOI
An autonomous folding unit mediates the assembly of two-stranded coiled coils
Richard A. Kammerer,Therese Schulthess,Ruth Landwehr,Ariel Lustig,Jürgen Engel,Ueli Aebi,Michel O. Steinmetz +6 more
TL;DR: A 13-residue sequence pattern that occurs with limited sequence variations in many two-stranded coiled coils and that is absolutely required for the assembly of the Dictyostelium discoideum actin-bundling protein cortexillin I and the yeast transcriptional activator GCN4 is reported.
Journal ArticleDOI
The broad‐spectrum potato cyst nematode resistance gene (Hero) from tomato is the only member of a large gene family of NBS‐LRR genes with an unusual amino acid repeat in the LRR region
TL;DR: The Hero gene of tomato is a broad spectrum resistance gene that confers a high level of resistance to all pathotypes of the potato cyst nematodes Globodera rostochiensis and partial resistance to G. pallida.
Journal ArticleDOI
Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs
Stefan Wiemann,Bernd Weil,Ruth Wellenreuther,Johannes Gassenhuber,Sabine Glassl,Wilhelm Ansorge,Michael Böcher,Helmut Blöcker,Stefan Bauersachs,Helmut Blum,Jürgen Lauber,A. Düsterhöft,Andreas Beyer,Karl Köhrer,Normann Strack,Hans-Werner Mewes,Birgit Ottenwälder,Brigitte Obermaier,Jens Tampe,Dagmar Heubner,R. Wambutt,Bernhard Korn,Michaela Klein,Annemarie Poustka +23 more
TL;DR: The sequencing and analysis of 500 novel human cDNAs containing the complete protein coding frame were reported, finding a number of genes that either had been completely missed in the analysis of the genomic sequences or had been wrongly predicted.
References
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Journal ArticleDOI
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
TL;DR: A 30-amino-acid segment of C/EBP, a newly discovered enhancer binding protein, shares notable sequence similarity with a segment of the cellular Myc transforming protein, and may represent a characteristic property of a new category of DNA binding proteins.
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Evidence that the leucine zipper is a coiled coil
TL;DR: A peptide corresponding to the leucine zipper region of the yeast transcriptional activator GCN4 was synthesized and characterized and associates in the micromolar concentration range to form a very stable dimer of alpha helices with a parallel orientation.
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Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.
TL;DR: The putative DNA-binding domain of CREB is structurally similar to the corresponding domains in the phorbol ester-responsive c-jun protein and the yeast transcription factor GCN4.
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α‐Helical coiled coils and bundles: How to design an α‐helical protein
Carolyn Cohen,David A.D. Parry +1 more
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A second class of synthetase structure revealed by X-ray analysis of Escherichia coli seryl-tRNA synthetase at 2.5 A.
TL;DR: The three-dimensional crystal structure of seryl-transfer RNA synthetase from Escherichia coli, refined at 2.5 Å resolution, is described, and is the first representative of a second class of aminoacyl-tRNA synthet enzyme structures.