Journal ArticleDOI
Predicting coiled coils from protein sequences
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TLDR
This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled -coil structures,such as the hinge region in myosin.Abstract:
The probability that a residue in a protein is part of a coiled-coil structure was assessed by comparison of its flanking sequences with sequences of known coiled-coil proteins. This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled-coil structures, such as the hinge region in myosin. More than 200 proteins that probably have coiled-coil domains were identified in GenBank, including alpha- and beta-tubulins, flagellins, G protein beta subunits, some bacterial transfer RNA synthetases, and members of the heat shock protein (Hsp70) family.read more
Citations
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Identification and characterization of a novel human plant pathogenesis-related protein that localizes to lipid-enriched microdomains in the Golgi complex
Heike B. Eberle,Ramon L Serrano,Joachim Füllekrug,Andreas Schlosser,Wolf D. Lehmann,Friedrich Lottspeich,Dora V. Kaloyanova,Felix T. Wieland,J. Bernd Helms +8 more
TL;DR: The identification of a novel family member that localizes to the cytosolic site of the endomembrane system in mammalian cells and myristoylation is reported, which could indicate that GAPR-1 is involved in the immune system.
Journal ArticleDOI
M phase phosphoprotein 1 is a human plus-end-directed kinesin-related protein required for cytokinesis.
Aouatef Abaza,Jean-Marc Soleilhac,Joanne M. Westendorf,Matthieu Piel,Isabelle Crevel,Aurélien Roux,Fabienne Pirollet +6 more
TL;DR: The human M phase phosphoprotein 1 (MPP1), previously identified through a screening of a subset of proteins specifically phosphorylated at the G2/M transition, is characterized as a plus-end-directed kinesin-related protein.
Journal ArticleDOI
Microtubule Organization Requires Cell Cycle-dependent Nucleation at Dispersed Cytoplasmic Sites: Polar and Perinuclear Microtubule Organizing Centers in the Plant Pathogen Ustilago maydis
TL;DR: The data suggest that MT reorganization in U. maydis depends on cell cycle-specific nucleation at dispersed cytoplasmic sites, at a polar MTOC and the SPB, which supports the notion that polar growth requires polar MT nucleation.
Journal ArticleDOI
Saccharomyces cerevisiae putative G protein, Gtr1p, which forms complexes with itself and a novel protein designated as Gtr2p, negatively regulates the Ran/Gsp1p G protein cycle through Gtr2p.
TL;DR: It is found that gtr1-11 caused a single amino acid substitution in Gtr1p, forming S20L, which is a putative, inactive GDP-bound mutant protein, which implies that Gtr 1p negatively regulates the Ran/Gsp1p GTPase cycle through Gtr2p.
Journal ArticleDOI
GTP binding induces filament assembly of a recombinant septin.
TL;DR: Re reconstituted filament assembly in vitro by using a recombinant Xenopus septin, Xl Sept2, and results imply that the growth or stability of septin filaments, or both, is regulated by the state of bound nucleotide.
References
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Journal ArticleDOI
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
TL;DR: A 30-amino-acid segment of C/EBP, a newly discovered enhancer binding protein, shares notable sequence similarity with a segment of the cellular Myc transforming protein, and may represent a characteristic property of a new category of DNA binding proteins.
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Evidence that the leucine zipper is a coiled coil
TL;DR: A peptide corresponding to the leucine zipper region of the yeast transcriptional activator GCN4 was synthesized and characterized and associates in the micromolar concentration range to form a very stable dimer of alpha helices with a parallel orientation.
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Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.
TL;DR: The putative DNA-binding domain of CREB is structurally similar to the corresponding domains in the phorbol ester-responsive c-jun protein and the yeast transcription factor GCN4.
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α‐Helical coiled coils and bundles: How to design an α‐helical protein
Carolyn Cohen,David A.D. Parry +1 more
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A second class of synthetase structure revealed by X-ray analysis of Escherichia coli seryl-tRNA synthetase at 2.5 A.
TL;DR: The three-dimensional crystal structure of seryl-transfer RNA synthetase from Escherichia coli, refined at 2.5 Å resolution, is described, and is the first representative of a second class of aminoacyl-tRNA synthet enzyme structures.