Journal ArticleDOI
Predicting coiled coils from protein sequences
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TLDR
This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled -coil structures,such as the hinge region in myosin.Abstract:
The probability that a residue in a protein is part of a coiled-coil structure was assessed by comparison of its flanking sequences with sequences of known coiled-coil proteins. This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled-coil structures, such as the hinge region in myosin. More than 200 proteins that probably have coiled-coil domains were identified in GenBank, including alpha- and beta-tubulins, flagellins, G protein beta subunits, some bacterial transfer RNA synthetases, and members of the heat shock protein (Hsp70) family.read more
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Characterization of a novel autophagy-specific gene, ATG29.
Tomoko Kawamata,Yoshiaki Kamada,Yoshiaki Kamada,Kuninori Suzuki,Kuninori Suzuki,Norihiro Kuboshima,Hiroshi Akimatsu,Shinichi Ota,Mariko Ohsumi,Yoshinori Ohsumi,Yoshinori Ohsumi +10 more
TL;DR: It is proposed that Atg29 functions in autophagosome formation at the pre-autophagosomal structure (PAS) in collaboration with other Atg proteins.
Journal ArticleDOI
A motif in human histidyl-tRNA synthetase which is shared among several aminoacyl-tRNA synthetases is a coiled-coil that is essential for enzymatic activity and contains the major autoantigenic epitope.
Nina Raben,Ralph C. Nichols,Jan Dohlman,Peter McPhie,Vaidehi Sridhar,C.Craig Hyde,Richard L. Leff,Paul H. Plotz +7 more
TL;DR: In this article, a recombinant histidyl-tRNA synthetase was found to be enzymatically active and recognized by human autoantibodies in the baculovirus system, and the peptides from this region (amino acids 1-60 and 1-47) have the predicted high alpha-helical content, but smaller fragments (1-30, 14-45, and 31-60) do not.
Journal ArticleDOI
Characterization of Unconventional MYO6, the Human Homologue of the Gene Responsible for Deafness in Snell's Waltzer Mice
Karen B. Avraham,Tama Hasson,Tama Sobe,Binaifer R. Balsara,Joseph R. Testa,Anne B. Skvorak,Cynthia C. Morton,Neal G. Copeland,Nancy A. Jenkins +8 more
TL;DR: The cloning and characterization of the human unconventional myosin VI (locus designation, MYO6) cDNA is reported, which makes this gene an excellent candidate for a human deafness disorder.
Journal ArticleDOI
REPPER--repeats and their periodicities in fibrous proteins.
TL;DR: FTwin and REPwin are complemented by secondary structure prediction (PSIPRED) and coiled coil prediction (COILS), making the server a versatile analysis tool for sequences of fibrous proteins.
Journal ArticleDOI
LIN-5 Is a Novel Component of the Spindle Apparatus Required for Chromosome Segregation and Cleavage Plane Specification in Caenorhabditis elegans
TL;DR: The results show that LIN-5 is a novel component of the spindle apparatus required for chromosome and spindle movements, cytoplasmic cleavage, and correct alternation of the S and M phases of the cell cycle.
References
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Journal ArticleDOI
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
TL;DR: A 30-amino-acid segment of C/EBP, a newly discovered enhancer binding protein, shares notable sequence similarity with a segment of the cellular Myc transforming protein, and may represent a characteristic property of a new category of DNA binding proteins.
Journal ArticleDOI
Evidence that the leucine zipper is a coiled coil
TL;DR: A peptide corresponding to the leucine zipper region of the yeast transcriptional activator GCN4 was synthesized and characterized and associates in the micromolar concentration range to form a very stable dimer of alpha helices with a parallel orientation.
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Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.
TL;DR: The putative DNA-binding domain of CREB is structurally similar to the corresponding domains in the phorbol ester-responsive c-jun protein and the yeast transcription factor GCN4.
Journal ArticleDOI
α‐Helical coiled coils and bundles: How to design an α‐helical protein
Carolyn Cohen,David A.D. Parry +1 more
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A second class of synthetase structure revealed by X-ray analysis of Escherichia coli seryl-tRNA synthetase at 2.5 A.
TL;DR: The three-dimensional crystal structure of seryl-transfer RNA synthetase from Escherichia coli, refined at 2.5 Å resolution, is described, and is the first representative of a second class of aminoacyl-tRNA synthet enzyme structures.