Journal ArticleDOI
Predicting coiled coils from protein sequences
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TLDR
This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled -coil structures,such as the hinge region in myosin.Abstract:
The probability that a residue in a protein is part of a coiled-coil structure was assessed by comparison of its flanking sequences with sequences of known coiled-coil proteins. This method was used to delineate coiled-coil domains in otherwise globular proteins, such as the leucine zipper domains in transcriptional regulators, and to predict regions of discontinuity within coiled-coil structures, such as the hinge region in myosin. More than 200 proteins that probably have coiled-coil domains were identified in GenBank, including alpha- and beta-tubulins, flagellins, G protein beta subunits, some bacterial transfer RNA synthetases, and members of the heat shock protein (Hsp70) family.read more
Citations
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Two microtubule-associated proteins required for anaphase spindle movement in Saccharomyces cerevisiae.
TL;DR: The analysis of the phenotype of a bik1 ase1 conditional double mutant suggests that BIK1 and ASE1 are not required for the assembly of a bipolar spindle, but are essential for anaphase spindle elongation.
Journal ArticleDOI
Getting the most from PSI-BLAST.
David T. Jones,Mark B. Swindells +1 more
TL;DR: Some of the key steps in getting the most from PSI-Blast, one of the most popular and powerful homology search programs currently available are explained.
Journal ArticleDOI
The Tousled gene in A. thaliana encodes a protein kinase homolog that is required for leaf and flower development
TL;DR: Using a mutation derived by T-DNA insertion mutagenesis, the TSL gene is cloned and it is found that it encodes a protein kinase homolog with a novel N-terminal domain that identifies a novel signaling/regulatory pathway used during development in Arabidopsis.
Journal ArticleDOI
Identification of PKDL, a novel polycystic kidney disease 2-like gene whose murine homologue is deleted in mice with kidney and retinal defects.
Hideki Nomura,Alberto Turco,York Pei,Luba Kalaydjieva,Tina Schiavello,Stanislawa Weremowicz,Weizhen Ji,Cynthia C. Morton,Miriam H. Meisler,Stephen T. Reeders,Jing Zhou +10 more
TL;DR: A new member of the polycystin protein family has been identified in this paper, which is a candidate candidate for as yet unmapped cystic diseases in man and animals.
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Determinants of kinesin motor polarity
TL;DR: The neck-motor junction was critical for Ncd minus-end movement; attachment of the neck to the stalk may also play a role; residues or regions contributing to motor polarity must be present in both the Ncd neck and the kinesin motor core.
References
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Journal ArticleDOI
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
TL;DR: A 30-amino-acid segment of C/EBP, a newly discovered enhancer binding protein, shares notable sequence similarity with a segment of the cellular Myc transforming protein, and may represent a characteristic property of a new category of DNA binding proteins.
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Evidence that the leucine zipper is a coiled coil
TL;DR: A peptide corresponding to the leucine zipper region of the yeast transcriptional activator GCN4 was synthesized and characterized and associates in the micromolar concentration range to form a very stable dimer of alpha helices with a parallel orientation.
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Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.
TL;DR: The putative DNA-binding domain of CREB is structurally similar to the corresponding domains in the phorbol ester-responsive c-jun protein and the yeast transcription factor GCN4.
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α‐Helical coiled coils and bundles: How to design an α‐helical protein
Carolyn Cohen,David A.D. Parry +1 more
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A second class of synthetase structure revealed by X-ray analysis of Escherichia coli seryl-tRNA synthetase at 2.5 A.
TL;DR: The three-dimensional crystal structure of seryl-transfer RNA synthetase from Escherichia coli, refined at 2.5 Å resolution, is described, and is the first representative of a second class of aminoacyl-tRNA synthet enzyme structures.