Proteome dynamics at broken replication forks reveal a distinct ATM-directed repair response suppressing DNA double-strand break ubiquitination
Kyosuke Nakamura,Georg Kustatscher,Constance Alabert,Martina Hödl,Ignasi Forné,Moritz Völker-Albert,Shankha Satpathy,Tracey E. Beyer,Niels Mailand,Chunaram Choudhary,Axel Imhof,Juri Rappsilber,Juri Rappsilber,Anja Groth +13 more
TLDR
In this article, the authors used nascent chromatin capture (NCC) proteomics to characterize the repair of replication-associated DNA double-strand breaks (DSBs) triggered by topoisomerase 1 (TOP1) inhibitors.About:
This article is published in Molecular Cell.The article was published on 2021-03-04 and is currently open access. It has received 37 citations till now. The article focuses on the topics: DNA repair & DNA replication.read more
Citations
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Talazoparib monotherapy in metastatic castration-resistant prostate cancer with DNA repair alterations (TALAPRO-1): an open-label, phase 2 trial
Johann S. de Bono,Niven Mehra,Giorgio V. Scagliotti,Elena Castro,Tanya B. Dorff,A. Stirling,Arnulf Stenzl,Mark D. Fleming,Celestia S. Higano,Fred Saad,Consuelo Buttigliero,Inge M. van Oort,A. Douglas Laird,Marielena Mata,Hsiang-Chun Chen,Cynthia G. Healy,Akos Czibere,Karim Fizazi +17 more
TL;DR: In this paper, the authors assessed the PARP inhibitor talazoparib in metastatic castration-resistant prostate cancers with DNA damage response (DDR) alterations in genes involved directly or indirectly in homologous recombination repair (HRR).
Journal ArticleDOI
Human topoisomerases and their roles in genome stability and organization
TL;DR: Topoisomerases are a family of six enzymes: TOP1 and TOP1MT, TOP2A and TOP2B, and two types IA and TOP3A as discussed by the authors .
Journal ArticleDOI
Cellular functions of the protein kinase ATM and their relevance to human disease.
Ji-Hoon Lee,Tanya T. Paull +1 more
TL;DR: The protein kinase ataxia telangiectasia mutated (ATM) is a master regulator of double-strand DNA break (DSB) signalling and stress responses as discussed by the authors.
Journal ArticleDOI
Hallmarks of DNA replication stress.
Sneha Saxena,Lee Zou +1 more
TL;DR: A review on the major sources of replication stress, the impacts of DNA replication stress in cells, and the assays to detect replication stress can be found in this paper , which provides an overview of the hallmarks of the replication stress.
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Present and Future Perspective on PLK1 Inhibition in Cancer Treatment
Michela Chiappa,Sabrina Petrella,Giovanna Damia,Massimo Broggini,Federica Guffanti,Francesco Ricci +5 more
TL;DR: The evidence suggesting the role of PLK1 in response to DNA damage, including DNA repair, cell cycle progression, epithelial to mesenchymal transition, cell death pathways and cancer-related immunity is summarized.
References
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Journal Article
Arrest of Replication Forks by Drug-stabilized Topoisomerase I-DNA Cleavable Complexes as a Mechanism of Cell Killing by Camptothecin
TL;DR: It is proposed that the collision between moving replication forks and camptothecin-stabilized topoisomerase I-DNA cleavable complexes results in fork arrest and possibly fork breakage, which are lethal to proliferating cells.
Journal ArticleDOI
Orchestration of the DNA-Damage Response by the RNF8 Ubiquitin Ligase
Nadine K. Kolas,J. Ross Chapman,Shinichiro Nakada,Jarkko Ylanko,Jarkko Ylanko,Richard Chahwan,Frédéric D. Sweeney,Frédéric D. Sweeney,Stephanie Panier,Megan Mendez,Jan Wildenhain,Timothy M. Thomson,Laurence Pelletier,Laurence Pelletier,Stephen P. Jackson,Daniel Durocher,Daniel Durocher +16 more
TL;DR: The results demonstrate how the DNA-damage response is orchestrated by ATM-dependent phosphorylation of MDC1 and RNF8-mediated ubiquitination and promote the G2/M DNA damage checkpoint and resistance to ionizing radiation.
Journal ArticleDOI
Hydroxyurea-Stalled Replication Forks Become Progressively Inactivated and Require Two Different RAD51-Mediated Pathways for Restart and Repair
Eva Petermann,Manuel Luis Orta,Manuel Luis Orta,Natalia Issaeva,Niklas Schultz,Thomas Helleday,Thomas Helleday +6 more
TL;DR: The XRCC3 protein, which is required for RAD51 foci formation, is also required for replication restart of HU-stalled forks, suggesting that RAD51-mediated strand invasion supports fork restart.
Journal ArticleDOI
Abraxas and RAP80 Form a BRCA1 Protein Complex Required for the DNA Damage Response
Bin Wang,Shuhei Matsuoka,Bryan A. Ballif,Bryan A. Ballif,Dong Zhang,Agata Smogorzewska,Steven P. Gygi,Stephen J. Elledge +7 more
TL;DR: Phosphopeptide affinity proteomic analysis identified a protein, Abraxas, that directly binds the BRCA1 BRCT repeats through a phospho-Ser-X- X-Phe motif, forming a third type of B RCA1 complex.
Journal ArticleDOI
RAP80 targets BRCA1 to specific ubiquitin structures at DNA damage sites.
Bijan Sobhian,Genze Shao,Dana R. Lilli,Aedín C. Culhane,Lisa A. Moreau,Bing Xia,David M. Livingston,Roger A. Greenberg +7 more
TL;DR: Mutations affecting the BRCT domains of the breast cancer–associated tumor suppressor BRCA1 disrupt the recruitment of this protein to DNA double-strand breaks (DSBs), implicating ubiquitin chain recognition and turnover in the BRCa1-mediated repair of DSBs.
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