Proteome dynamics at broken replication forks reveal a distinct ATM-directed repair response suppressing DNA double-strand break ubiquitination
Kyosuke Nakamura,Georg Kustatscher,Constance Alabert,Martina Hödl,Ignasi Forné,Moritz Völker-Albert,Shankha Satpathy,Tracey E. Beyer,Niels Mailand,Chunaram Choudhary,Axel Imhof,Juri Rappsilber,Juri Rappsilber,Anja Groth +13 more
TLDR
In this article, the authors used nascent chromatin capture (NCC) proteomics to characterize the repair of replication-associated DNA double-strand breaks (DSBs) triggered by topoisomerase 1 (TOP1) inhibitors.About:
This article is published in Molecular Cell.The article was published on 2021-03-04 and is currently open access. It has received 37 citations till now. The article focuses on the topics: DNA repair & DNA replication.read more
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Multi-layered chromatin proteomics identifies cell vulnerabilities in DNA repair
TL;DR: In this article , the authors employ multi-layered proteomics to characterize chromatin-mediated functional interactions of repair proteins, signatures of hPTMs and the DNA-bound proteome during DNA double-strand break (DSB) repair at high temporal resolution.
Journal ArticleDOI
Histone dynamics during DNA replication stress
TL;DR: In this article, the authors summarize the current understanding of histone dynamics in replication stress, highlighting recent advances in the characterization of fork-protective mechanisms and uncover links between defects in replication stresses responses and genome instability or various diseases, such as cancer.
Journal ArticleDOI
Autologous K63 deubiquitylation within the BRCA1-A complex licenses DNA damage recognition
Qinqin Jiang,Martina Foglizzo,Yaroslav I. Morozov,Xuejiao Yang,Arindam Datta,Lei Tian,Vaughn Thada,Weihua Li,Elton Zeqiraj,Roger A. Greenberg +9 more
TL;DR: Findings identify RAP80 and BRCC36 as autologous DUB substrates in the BRCA1-A complex, thus explaining the evolution of matching ubiquitin-binding and hydrolysis activities within a single macromolecular assembly.
Posted ContentDOI
Multi-layered chromatin proteomics identifies cell vulnerabilities in DNA repair
Gianluca Sigismondo,Lavinia Arseni,Thomas G. Hofmann,Martina Seiffert,Jeroen Krijgsveld,Jeroen Krijgsveld +5 more
TL;DR: Vardef et al. as mentioned in this paper employed multi-layered proteomics to characterize chromatin mediated interactions of repair proteins, signatures of hPTMs, and the DNA-bound proteome during DNA double-strand break repair at high temporal resolution.
Journal ArticleDOI
Complementary CRISPR genome-wide genetic screens in PARP10-knockout and overexpressing cells identify synthetic interactions for PARP10-mediated cellular survival
Jude B. Khatib,Emily M. Schleicher,L. Murl Jackson,Ashna Dhoonmoon,George Lucian Moldovan,Claudia M. Nicolae +5 more
TL;DR: It is found that PARP10-overexpressing cells rely on multiple DNA repair genes for survival, including ATM, the master regulator of the DNA damage checkpoint, and the CDK2-Cyclin E1 complex is identified as essential for proliferation of PARP 10-knockout cells.
References
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Paul Shannon,Andrew Markiel,Owen Ozier,Nitin S. Baliga,Jonathan T. Wang,Daniel Ramage,Nada Amin,Benno Schwikowski,Trey Ideker +8 more
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The PRIDE database and related tools and resources in 2019: improving support for quantification data.
Yasset Perez-Riverol,Attila Csordas,Jingwen Bai,Manuel Bernal-Llinares,Suresh Hewapathirana,Deepti J. Kundu,Avinash Inuganti,Johannes Griss,Johannes Griss,Gerhard Mayer,Martin Eisenacher,Enrique Perez,Julian Uszkoreit,Julianus Pfeuffer,Timo Sachsenberg,Şule Yılmaz,Shivani Tiwary,Juergen Cox,Enrique Audain,Mathias Walzer,Andrew F. Jarnuczak,Tobias Ternent,Alvis Brazma,Juan Antonio Vizcaíno +23 more
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