Replication Fork Stalling at Natural Impediments
TLDR
This review describes the mechanisms and consequences of replication stalling at various natural impediments, with an emphasis on the role of replicationStalling in genomic instability.Abstract:
Summary: Accurate and complete replication of the genome in every cell division is a prerequisite of genomic stability. Thus, both prokaryotic and eukaryotic replication forks are extremely precise and robust molecular machines that have evolved to be up to the task. However, it has recently become clear that the replication fork is more of a hurdler than a runner: it must overcome various obstacles present on its way. Such obstacles can be called natural impediments to DNA replication, as opposed to external and genetic factors. Natural impediments to DNA replication are particular DNA binding proteins, unusual secondary structures in DNA, and transcription complexes that occasionally (in eukaryotes) or constantly (in prokaryotes) operate on replicating templates. This review describes the mechanisms and consequences of replication stalling at various natural impediments, with an emphasis on the role of replication stalling in genomic instability.read more
Citations
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ATR: an essential regulator of genome integrity
Karlene A. Cimprich,David Cortez +1 more
TL;DR: New insights are provided into the mechanisms that control ATR activation, which have helped to explain the overlapping but non-redundant activities of ATR and ATM in DNA-damage signalling, and have clarified the crucial functions of AtR in maintaining genome integrity.
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R Loops: From Transcription Byproducts to Threats to Genome Stability
TL;DR: The factors and cellular processes that control R loop formation and the mechanisms by which R loops may influence gene expression and the integrity of the genome are discussed.
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Maintaining genome stability at the replication fork.
Dana Branzei,Marco Foiani +1 more
TL;DR: These mechanisms ensure that the local DNA damage response, which enables replication fork progression and DNA repair in S phase, is coupled with cell cycle transitions.
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Sources of DNA Double-Strand Breaks and Models of Recombinational DNA Repair
Anuja Mehta,James E. Haber +1 more
TL;DR: In this paper, the authors highlight the chief sources of double-strand break (DSB) and crucial requirements for each of these repair processes, as well as the methods to identify and study intermediate steps in DSB repair by homologous recombination.
Journal ArticleDOI
Replication Fork Stalling at Natural Impediments
TL;DR: This review describes the mechanisms and consequences of replication stalling at various natural impediments, with an emphasis on the role of replicationStalling in genomic instability.
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Comprehensive Identification of Cell Cycle–regulated Genes of the Yeast Saccharomyces cerevisiae by Microarray Hybridization
Paul T. Spellman,Gavin Sherlock,Gavin Sherlock,Michael Q. Zhang,Vishwanath R. Iyer,Kirk R. Anders,Michael B. Eisen,Patrick O. Brown,Patrick O. Brown,David Botstein,Bruce Futcher +10 more
TL;DR: A comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle is created, and it is found that the mRNA levels of more than half of these 800 genes respond to one or both of these cyclins.