Retinal Ganglion Cell Transplantation: Approaches for Overcoming Challenges to Functional Integration
TLDR
The current state of experimental RGC transplantation is summarized in this paper, and a set of standard approaches to quantifying and reporting experimental outcomes is proposed to guide a collective effort toward functional RGC replacement and optic nerve regeneration.Abstract:
As part of the central nervous system, mammalian retinal ganglion cells (RGCs) lack significant regenerative capacity. Glaucoma causes progressive and irreversible vision loss by damaging RGCs and their axons, which compose the optic nerve. To functionally restore vision, lost RGCs must be replaced. Despite tremendous advancements in experimental models of optic neuropathy that have elucidated pathways to induce endogenous RGC neuroprotection and axon regeneration, obstacles to achieving functional visual recovery through exogenous RGC transplantation remain. Key challenges include poor graft survival, low donor neuron localization to the host retina, and inadequate dendritogenesis and synaptogenesis with afferent amacrine and bipolar cells. In this review, we summarize the current state of experimental RGC transplantation, and we propose a set of standard approaches to quantifying and reporting experimental outcomes in order to guide a collective effort to advance the field toward functional RGC replacement and optic nerve regeneration.read more
Citations
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Journal ArticleDOI
Outer Retinal Cell Replacement: Putting the Pieces Together.
Allison L. Ludwig,David M. Gamm +1 more
TL;DR: A review of the history of neuroretinal and photoreceptors (PR) cell transplantation can be found in this paper, where a stepwise approach is proposed to address specific pieces of the outer retinal cell replacement puzzle.
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ICG-mediated photodisruption of the inner limiting membrane enhances retinal drug delivery.
Karen Peynshaert,Helena Vanluchene,Kaat De Clerck,An-Katrien Minnaert,Morgane Verhoeven,Noémie Gouspillou,Nezahat Bostan,Toshio Hisatomi,Geraldine Accou,Félix Sauvage,Kevin Braeckmans,Stefaan C. De Smedt,Katrien Remaut +12 more
TL;DR: In this article , a light-based approach to locally ablate the inner limiting membrane (ILM) in a minimally invasive and highly controlled manner, thus making the ILM more permeable for therapeutics was explored.
Journal ArticleDOI
Analyses of transplanted human retinal ganglion cell morphology and localization in murine organotypic retinal explant culture
Kevin Y. Zhang,Thomas V. Johnson +1 more
TL;DR: Zhang et al. as mentioned in this paper presented a protocol for ex vivo human RGC transplantation on flatmounted murine organotypic retinal explants, providing a robust platform for studying donor RGC survival, dendritic stratification, topographic distribution, donor-host interactions, and pro-engraftment strategies.
Journal ArticleDOI
Photodisruption of the Inner Limiting Membrane: Exploring ICG Loaded Nanoparticles as Photosensitizers
Kaat De Clerck,Geraldine Accou,Félix Sauvage,Kevin Braeckmans,Stefaan C. De Smedt,Katrien Remaut,Karen Peynshaert +6 more
TL;DR: Application of ICG liposomes followed by laser treatment resulted in subtle disruption effects at the ILM where zones of fully ablated ILM were alternated by intact regions, improving the concept to a further extent.
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Re-formation of synaptic connectivity in dissociated human stem cell-derived retinal organoid cultures
Allison L. Ludwig,Steven J. Mayerl,Yu Gao,Mark Banghart,Cole Bacig,Maria A Fernandez Zepeda,Xinyu Zhao,David M. Gamm +7 more
TL;DR: In this paper , the authors employed a highly controlled, rabies virus-based monosynaptic tracing assay to investigate neuronal reconnection following dissociation of early RO cultures, which provided evidence for synaptic plasticity of RO-derived retinal neurons.
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