Journal ArticleDOI
Rotavirus genotyping: keeping up with an evolving population of human rotaviruses
TLDR
Methods and oligonucleotide primers are described to overcome failures to type G9, G 10 and P[11] rotavirus strains, and cross-reactivity identified between G10 and G3 rotaviruses.About:
This article is published in Journal of Clinical Virology.The article was published on 2004-12-01. It has received 411 citations till now. The article focuses on the topics: Rotavirus & Reassortment.read more
Citations
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Journal ArticleDOI
Virus hazards from food, water and other contaminated environments.
David Rodríguez-Lázaro,Nigel Cook,Franco Maria Ruggeri,Jane Sellwood,Abid Nasser,Maria São José Nascimento,Martin D'Agostino,Ricardo Santos,Juan-Carlos Saiz,Artur Rzeżutka,Albert Bosch,Rosina Girones,Annalaura Carducci,Michelle Muscillo,Katarina Kovač,Marta Diez-Valcarce,Apostolos Vantarakis,Carl-Henrik von Bonsdorff,Ana Maria de Roda Husman,Marta Hernández,Wim H. M. van der Poel,Wim H. M. van der Poel +21 more
TL;DR: M Molecular epidemiology and surveillance of environmental samples are necessary to elucidate the public health hazards associated with exposure to environmental viruses and the human pathogenic viruses that are most relevant in this context are nonenveloped.
Journal ArticleDOI
Efficacy of a monovalent human-bovine (116E) rotavirus vaccine in Indian infants: a randomised, double-blind, placebo-controlled trial.
Nita Bhandari,Temsunaro Rongsen-Chandola,Ashish Bavdekar,Jacob John,Kalpana Antony,Sunita Taneja,Nidhi Goyal,Anand Kawade,Gagandeep Kang,Sudeep Singh Rathore,Sanjay Juvekar,Jayaprakash Muliyil,Alok Arya,Hanif Shaikh,Vinod Joseph Abraham,Sudhanshu Vrati,Michael A. Proschan,Robert Kohberger,Georges Thiry,Roger I. Glass,Harry B. Greenberg,George Curlin,Krishna Mohan,G.V.J.A. Harshavardhan,Sai Prasad,T.S. Rao,John W. Boslego,Maharaj K. Bhan +27 more
TL;DR: Monovalent human-bovine (116E) rotavirus vaccine is effective and well tolerated in Indian infants, and prevalence of immediate, solicited, and serious adverse events was similar in both groups.
Journal ArticleDOI
Rotavirus genotypes co-circulating in Europe between 2006 and 2009 as determined by EuroRotaNet, a pan-European collaborative strain surveillance network
Miren Iturriza-Gomara,Timothy J. Dallman,Krisztián Bányai,Blenda Böttiger,J. Buesa,S. Diedrich,Lucia Fiore,Kirsten L. Johansen,Marion Koopmans,Neli Korsun,D. Koukou,Annelies Kroneman,Brigitta László,Maija Lappalainen,Leena Maunula,A. Mas Marques,Jelle Matthijnssens,Sofie Midgley,Zornitsa Mladenova,Sameena Nawaz,Mateja Poljšak-Prijatelj,Pierre Pothier,Franco Maria Ruggeri,Alicia Sánchez-Fauquier,Andrej Steyer,I. Sidaraviciute-Ivaskeviciene,V. Syriopoulou,A. N. Tran,Vytautas Usonis,M. Van Ranst,A. De Rougemont,Jim Gray +31 more
TL;DR: The ability of the network to identify strains circulating with an incidence of ⩾1% allowed the identification of possible emerging strains such as G8 and G12 since the beginning of the study; analysis of recent data indicates their increased incidence.
Journal ArticleDOI
Stem Cell-Derived Human Intestinal Organoids as an Infection Model for Rotaviruses
TL;DR: Induced human intestinal organoids are a promising new model for generating intestine-like tissue and the evidence that both laboratory and clinical rotavirus isolates can replicate in iHIOs suggests that this model would be useful not only for studies of rotaviruses but also potentially of other infectious agents.
Journal ArticleDOI
Protective Effect of Natural Rotavirus Infection in an Indian Birth Cohort
Beryl Primrose Gladstone,Sasirekha Ramani,Indrani Mukhopadhya,Jayaprakash Muliyil,P. H. Rajiv Sarkar,Andrea M. Rehman,Shabbar Jaffar,Miren Iturriza Gómara,Jim Gray,David Brown,Ulrich Desselberger,Sue E. Crawford,Jacob John,Sudhir Babji,Mary K. Estes,Gagandeep Kang +15 more
TL;DR: Early infection and frequent reinfection in a locale with high viral diversity resulted in lower protection than has been reported elsewhere, providing a possible explanation why rotavirus vaccines have had lower-than-expected efficacy in Asia and Africa.
References
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Journal ArticleDOI
Rapid and simple method for purification of nucleic acids.
R. Boom,C. J. A. Sol,M. M. M. Salimans,C. L. Jansen,P.M.E. Wertheim-van Dillen,J. van der Noordaa +5 more
TL;DR: A simple, rapid, and reliable protocol for the small-scale purification of DNA and RNA from, e.g., human serum and urine, based on the lysing and nuclease-inactivating properties of guanidinium thiocyanate together with the nucleic acid-binding properties of silica particles or diatoms in the presence of this agent.
Journal ArticleDOI
Global Illness and Deaths Caused by Rotavirus Disease in Children
TL;DR: The tremendous incidence of rotavirus disease underscores the urgent need for interventions, such as vaccines, to prevent childhood deaths in developing nations.
Journal ArticleDOI
Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens.
Vera Gouvea,Roger I. Glass,P. A. Woods,Koki Taniguchi,H. F. Clark,Barbara Forrester,Zhao-Yin Fang +6 more
TL;DR: A PCR typing method was devised in which each human serotype virus produced a characteristic segment size, readily identifiable in agarose gels, which provided a rapid and efficient means of obtaining large quantities of cDNA suitable for sequencing, cloning, and other genetic studies, precluding the need for cell culture and virus purification.
Journal ArticleDOI
Identification of group A rotavirus gene 4 types by polymerase chain reaction.
Jon R. Gentsch,Roger I. Glass,P. A. Woods,Vera Gouvea,M Gorziglia,J Flores,Bimal Kumar Das,Maharaj K. Bhan +7 more
TL;DR: The results suggest that gene 4 typing will be useful in providing more a complete characterization of HRV strains of epidemiologic or vaccine-related interest.
Journal ArticleDOI
Characterization of rotavirus strains from newborns in New Delhi, India.
Bimal Kumar Das,J. R. Gentsch,H G Cicirello,P. A. Woods,A. Gupta,Manoj Ramachandran,Rajive Kumar,Maharaj K. Bhan,Roger I. Glass +8 more
TL;DR: Between 1986 and 1993, 72% of rotavirus strains isolated from newborns at five hospitals in New Delhi, India, had long electropherotypes, subgroup II VP6 antigens, and G and P genotypes identical to those of prototype strain 116E.
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